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About: Inhalation of infectious bioaerosols has been linked to a variety of respiratory diseases. However, efficient sampling techniques to allow high temporal resolution sampling are limited to collect and study bioaerosols in the various occupational and ambient micro–environmental atmospheres. This study introduces a medium flow swirling bioaerosol sampler (SAS) approach that collects atmospheric bioaerosols at the flow rate of 167 Lpm (10 cubic meter per hour). The collection of bioaerosols is achieved through a combination of impaction and cyclonic centrifugal motion. Aerosol deposition efficiency tests were performed with monodispersive polystyrene latex (PSL) particles ranging from 0.1 to 10 μm. Results have shown that the sampler has cut–off size of 0.7 μm and 1.5 μm, with and without the assistance of added water vapor, respectively. The bioaerosol collection and viability tests were performed with comparison to the commercially–available BioSampler, and the results show that the collection efficiency of the SAS is 97% at the designed flow rate, while the higher flow of the new system yields more than 13 times of the collection rate compared to the BioSampler. The high collection efficiency and observed viability preservation of the SAS make it an attractive alternative for high time resolution bioaerosol sampling for atmospheric, occupational and indoor air quality monitoring.

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