About: Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three domain structure for the nucleocapsid protein

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About: Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three domain structure for the nucleocapsid protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three domain structure for the nucleocapsid protein
Creator	Masters' Wadsworth, Paul Parker, Monica
Source	Elsevier; Medline; PMC
abstract	Abstract To obtain information about the structure and evolution of the nucleocapsid (N) protein of the coronavirus mouse hepatitis virus (MHV), we determined the entire nucleotide sequences of the N genes of MHV-A59, MHV-3, MHV-S, and MHV-1 from cDNA clones. At the nucleotide level, the N gene sequences of these viral strains, and that of MHV-JHM, were more than 92% conserved overall. Even higher nucleotide sequence identity was found in the 3′ untranslated regions (3′ UTRs) of the five strains, which may reflect the role of the 3′ UTR in negative-strand RNA synthesis. All five N genes were found to encode markedly basic proteins of 454 or 455 residues having at least 94% sequence identity in pairwise comparisons. However, amino acid sequence divergences were found to be clustered in two short segments of N, putative spacer regions that, together, constituted only 11% of the molecule. Thus, the data suggest that the MHV N protein is composed of three highly conserved structural domains connected to each other by regions that have much less constraint on their amino acid sequences. The first two conserved domains contain most of the excess of basic amino acid residues; by contrast, the carboxy-terminal domain is acidic. Finally, we noted that four of the five N genes contain an internal open reading frame that potentially encodes a protein of 207 amino acids having a large proportion of basic and hydrophobic residues.
has issue date	1990-11-30(xsd:dateTime)
bibo:doi	10.1016/0042-6822(90)90316-j
bibo:pmid	2171216
has license	els-covid
sha1sum (hex)	5d9708a84c8f53a508621c06001b9fb51a4bda3e
schema:url	https://doi.org/10.1016/0042-6822%2890%2990316-j
resource representing a document's title	Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three domain structure for the nucleocapsid protein
has PubMed Central identifier	PMC7131444
has PubMed identifier	2171216
schema:publication	Virology
resource representing a document's body	covid:5d9708a84c8f53a508621c06001b9fb51a4bda3e#body_text
is schema:about of	named entity 'Coronavirus' named entity 'Virus' named entity 'STRAINS' named entity 'SEQUENCE COMPARISON' named entity 'MOUSE HEPATITIS VIRUS' named entity 'CORONAVIRUS' named entity 'GENES' named entity 'DOMAIN STRUCTURE' named entity 'NUCLEOCAPSID PROTEIN' named entity 'Coronavirus' named entity 'Coronaviruses' named entity 'clones' named entity 'MHV' named entity 'clone' named entity 'translation initiation' named entity 'ORF' named entity 'recombination' named entity 'electrophoretic mobility' named entity 'gene' named entity '3' UTRs' named entity 'start codon' named entity 'gene sequences' named entity 'MHV' named entity 'sequence identity' named entity 'reading frame' named entity 'nucleocapsid' named entity 'reverse transcriptase' named entity 'A59' named entity 'MHV' named entity 'gene sequences' named entity 'MHV' named entity 'post-translational modification' named entity 'open reading frame' named entity 'nucleocapsid' named entity 'conserved domains' named entity 'SDS-PAGE' named entity 'cDNA clones' named entity 'nucleotides' named entity 'coronavirus' named entity 'amino acids' named entity '10.4' named entity 'protein' named entity '3' untranslated region' named entity 'protein' named entity 'nucleocapsid' named entity 'kDa' named entity 'tissue culture' named entity 'nucleocapsid' named entity 'A59' named entity 'RNA synthesis' named entity 'nucleotide sequences' named entity 'nucleotide sequences' named entity 'nucleocapsid' named entity 'nucleocapsid' named entity 'RNA-dependent RNA polymerase' named entity 'RNA viruses' named entity 'negative-strand' named entity 'MHV' named entity 'serine' named entity 'RNA synthesis' named entity 'ORF' named entity 'nucleocapsid' named entity 'MHV' named entity 'clone' named entity 'molecular biological' named entity 'protein' named entity 'coronavirus' named entity 'protein' named entity 'viral infection' named entity 'nucleocapsid' named entity 'nucleotide sequences' named entity 'secondary structure' named entity 'clone' named entity 'clones' named entity 'gene' named entity 'gene' named entity 'amino acid sequences' named entity 'start codon' named entity 'MHV' named entity 'MHV'

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