About: Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2

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About: Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2
Creator	Sun, F Aydin, M Bashir, R Berger, J Cunningham, B Ganguli, A King, W Mostafa, A Valera, E
Source	BioRxiv; Medline; PMC
abstract	The COVID-19 pandemic provides an urgent example where a gap exists between availability of state-of-the-art diagnostics and current needs. As assay details and primer sequences become widely known, many laboratories could perform diagnostic tests using methods such as RT-PCR or isothermal RT-LAMP amplification. A key advantage of RT-LAMP based approaches compared to RT-PCR is that RT-LAMP is known to be robust in detecting targets from unprocessed samples. In addition, RT-LAMP assays are performed at a constant temperature enabling speed, simplicity, and point-of-use testing. Here, we provide the details of an RT-LAMP isothermal assay for the detection of SARS-CoV-2 virus with performance comparable to currently approved tests using RT-PCR. We characterize the assay by introducing swabs in virus spiked synthetic nasal fluids, moving the swab to viral transport medium (VTM), and using a volume of that VTM for performing the amplification without an RNA extraction kit. The assay has a Limit-of-Detection (LOD) of 50 RNA copies/μL in the VTM solution within 20 minutes, and LOD of 5000 RNA copies/μL in the nasal solution. Additionally, we show the utility of this assay for real-time point-of-use testing by demonstrating detection of SARS-CoV-2 virus in less than 40 minutes using an additively manufactured cartridge and a smartphone-based reader. Finally, we explore the speed and cost advantages by comparing the required resources and workflows with RT-PCR. This work could accelerate the development and availability of SARS-CoV-2 diagnostics by proving alternatives to conventional laboratory benchtop tests.
has issue date	2020-05-21(xsd:dateTime)
bibo:doi	10.1101/2020.05.21.108381
bibo:pmid	32511358
has license	cc-by-nc-nd
sha1sum (hex)	47d722d59354dfee7b1aa9a2be5b696970f1ab8e
schema:url	https://doi.org/10.1101/2020.05.21.108381
resource representing a document's title	Rapid Isothermal Amplification and Portable Detection System for SARS-CoV-2
has PubMed Central identifier	PMC7263486
has PubMed identifier	32511358
schema:publication	bioRxiv
resource representing a document's body	covid:47d722d59354dfee7b1aa9a2be5b696970f1ab8e#body_text
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