About: LincRNA-p21 promotes mesenchymal stem cell migration capacity and survival through hypoxic preconditioning

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About: LincRNA-p21 promotes mesenchymal stem cell migration capacity and survival through hypoxic preconditioning Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	LincRNA-p21 promotes mesenchymal stem cell migration capacity and survival through hypoxic preconditioning
Creator	Yang, Yi Liu, Ling Qiu, Hai-Bo Chang, Wei Guo, Feng-Mei Huang, Li-Li Lu, Zhong-Hua Meng, Shan-Shan Xu, Jing-Yuan Xu, Xiu-Ping
Source	PMC
abstract	BACKGROUND: Mesenchymal stem cells (MSCs) derived from bone marrow have potent stabilizing effects for the treatment of acute respiratory distress syndrome (ARDS). However, low efficiency and survival in MSC homing to injured lung tissue remains to be solved. Therefore, the aim of this study was to assess whether large intergenic noncoding RNA (LincRNA)-p21 promote MSC migration and survival capacity through hypoxic preconditioning in vitro. METHODS: MSCs were cultured and divided into the normoxia culture group (20% O2) and hypoxia culture group (1% O2). To determine roles and mechanisms, lentivirus vector-mediated LincRNA-p21 knockdown of MSCs and hypoxia-inducible factor (HIF-1α) inhibitor KC7F2 were introduced. Additionally, MSC migration was analyzed by scratch test and transwell migration assays. MSC proliferation was tested by cell counting kit-8 and trypan blue dye. Apoptosis was detected by Annexin V-PE/7-AAD stained flow cytometry. Moreover, LincRNA-p21 and HIF-1α mRNA was measured by reverse transcription-polymerase chain reaction, and HIF-1α and CXCR4/7 protein were assayed by western blot (WB) or enzyme-linked immunosorbent assay (ELISA). Apoptosis protein caspase-3 and cleaved-caspase-3 were investigated by WB analysis. Considering interactions between VHL and HIF-1α under LincRNA-p21 effect, co-immunoprecipitation was detected. RESULTS: Hypoxic preconditioning MSC promoted migration capacity and MSC survival than normoxia culture group. MSCs induced by hypoxic preconditioning evoked an increase in expression of LincRNA-p21, HIF-1α, and CXCR4/7(both were chemokine stromal-derived factor-1(SDF-1) receptors). Contrarily, blockade of LincRNA-p21 by shRNA and HIF-1α inhibitor KC7F2 abrogated upregulation of hypoxic preconditioning induced CXCR4/7 in MSCs, cell migration, and survival. Furthermore, co-immunoprecipitation assay revealed that hypoxic preconditioning isolated VHL and HIF-1α protein by increasing HIF-1α expression. CONCLUSIONS: Hypoxic preconditioning was identified as a promoting factor of MSC migration and survival capacity. LincRNA-p21 promotes MSC migration and survival capacity through HIF-1α/CXCR4 and CXCR7 pathway under hypoxic preconditioning in vitro.
has issue date	2018-10-25(xsd:dateTime)
bibo:doi	10.1186/s13287-018-1031-x
bibo:pmid	30359325
has license	cc-by
sha1sum (hex)	1049562c838f202553d43127e68abe8a2f9d8fea
schema:url	https://doi.org/10.1186/s13287-018-1031-x
resource representing a document's title	LincRNA-p21 promotes mesenchymal stem cell migration capacity and survival through hypoxic preconditioning
has PubMed Central identifier	PMC6202870
has PubMed identifier	30359325
schema:publication	Stem Cell Res Ther
resource representing a document's body	covid:1049562c838f202553d43127e68abe8a2f9d8fea#body_text
is schema:about of	named entity 'Mesenchymal stem cells' named entity 'INTERGENIC' named entity 'CAPACITY' covid:arg/1049562c838f202553d43127e68abe8a2f9d8fea named entity 'However' named entity 'mesenchymal stem cell' named entity 'p21' named entity 'ARDS' named entity 'noncoding RNA' named entity 'mesenchymal stem cell' named entity 'paracrine' named entity 'HIF-1α' named entity 'western blot' named entity 'trypan blue' named entity 'mice' named entity 'HIF-1α' named entity 'MSC' named entity 'angiogenesis' named entity 'normoxic' named entity 'MSCs' named entity 'antisense' named entity 'MSC' named entity 'caspase-3' named entity 'pore size' named entity 'endocrine' named entity 'growth factors' named entity 'Reverse transcriptase' named entity 'post-transcriptional regulation' named entity 'hypoxic' named entity 'Vector NTI' named entity 'DMEM' named entity 'hypoxia' named entity 'HIF-1α' named entity 'Peroxidase' named entity 'p21' named entity 'HIF' named entity 'Cell Signaling' named entity 'LincRNA' named entity 'LincRNA' named entity 'MSC' named entity 'HIF-1α' named entity 'hypoxic' named entity 'primers' named entity 'LincRNA' named entity 'hypoxic' named entity 'LincRNA' named entity 'MSC' named entity 'MSC' named entity 'crystal violet' named entity 'MSC' named entity 'MSCs' named entity 'protein' named entity 'MSCs' named entity 'hypoxia' named entity 'LincRNA' named entity '1:250' named entity 'Cell Signaling' named entity '37°C' named entity 'HIF-1α' named entity 'dsDNA' named entity 'primary antibodies' named entity 'cell cycle' named entity 'animal experiment' named entity 'CXCR4' named entity 'Cell counts' named entity 'MSC' named entity 'transcriptional' named entity 'Cell Counting' named entity 'staining' named entity 'mRNA' named entity 'trypan blue' named entity 'MSC'

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