About: Target-independent high-throughput sequencing methods provide evidence that already known human viral pathogens play a main role in respiratory infections with unexplained etiology

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About: Target-independent high-throughput sequencing methods provide evidence that already known human viral pathogens play a main role in respiratory infections with unexplained etiology Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Target-independent high-throughput sequencing methods provide evidence that already known human viral pathogens play a main role in respiratory infections with unexplained etiology
Creator	Casas, Inmaculada Calvo, Cristina Pozo, Francisco Palacios, Gustavo Wiley, Michael Prieto, Karla Iglesias-Caballero, María Pérez-Sautu, Unai Chitty, Joseph Luz García-García, María
Source	Medline; PMC
abstract	Despite the advanced PCR-based assays available, a fraction of the pediatric respiratory infections remain unexplained every epidemic season, and there is a perception that novel viruses might be present in these specimens. We systematically collected samples from a prospective cohort of pediatric patients with respiratory infections, that returned negative results by validated molecular RT–PCR assays, and studied them with a target-independent, high-throughput sequencing-based approach. We also included a matched cohort of children with no symptoms of respiratory infection, as a contrast study population. More than fifty percent of the specimens from the group of patients with unexplained respiratory infections were resolved. However, the higher rate of detection was not due to the presence of novel viruses, but to the identification of well-known viral respiratory pathogens. Our results show that already known viral pathogens are responsible for the majority of cases that remain unexplained after the epidemic season. High-throughput sequencing approaches that use pathogen-specific probes are easier to standardize because they ensure reproducible library enrichment and sequencing. In consequence, these techniques might be desirable from a regulatory standpoint for diagnostic laboratories seeking to benefit from the many advantages of these sequencing technologies.
has issue date	2019-07-23(xsd:dateTime)
bibo:doi	10.1080/22221751.2019.1640587
bibo:pmid	31335277
has license	cc-by
sha1sum (hex)	6d76fbc0763d467e9109feefe43e4e52113b78f3
schema:url	https://doi.org/10.1080/22221751.2019.1640587
resource representing a document's title	Target-independent high-throughput sequencing methods provide evidence that already known human viral pathogens play a main role in respiratory infections with unexplained etiology
has PubMed Central identifier	PMC6691886
has PubMed identifier	31335277
schema:publication	Emerg Microbes Infect
resource representing a document's body	covid:6d76fbc0763d467e9109feefe43e4e52113b78f3#body_text
is schema:about of	named entity 'probes' named entity 'desirable' named entity 'sequencing technologies' named entity 'library' named entity 'patients' named entity 'advanced' named entity 'cohort' named entity 'RESPIRATORY INFECTIONS' named entity 'ROLE' named entity 'MOLECULAR' named entity 'USE' covid:arg/6d76fbc0763d467e9109feefe43e4e52113b78f3 named entity 'TARGET' named entity 'PCR' named entity 'ENSURE' named entity 'DETECTION' named entity 'APPROACHES' named entity 'THESE' named entity 'FIFTY' named entity 'TARGET' named entity 'ENRICHMENT' named entity 'PERCEPTION' named entity 'REGULATORY' named entity 'NO SYMPTOMS' named entity 'CASES' named entity 'SEASON' named entity 'RATE' named entity 'IS A' named entity 'SEQUENCING' named entity 'UNEXPLAINED' named entity 'AVAILABLE' named entity 'PEDIATRIC' named entity 'PLAY' named entity 'HIGH-THROUGHPUT SEQUENCING' named entity 'INDEPENDENT' named entity 'EVIDENCE' named entity 'ETIOLOGY' named entity 'UNEXPLAINED' named entity 'BENEFIT' named entity 'PATIENTS' named entity 'PERCENT' named entity 'STUDY POPULATION' named entity 'VIRAL' named entity 'PROBES' named entity 'TECHNIQUES' named entity 'HUMAN' named entity 'METHODS' named entity 'KNOWN' named entity 'MAIN' named entity 'VIRAL PATHOGENS' named entity 'VALIDATED' named entity 'HIGH-THROUGHPUT SEQUENCING' named entity 'APPROACH' named entity 'COLLECTED' named entity 'MAJORITY' named entity 'GROUP OF PATIENTS' named entity 'OUR' named entity 'INDEPENDENT' named entity 'PATHOGEN' named entity 'KNOWN' named entity 'FRACTION OF' named entity 'BASED' named entity 'HIGHER' named entity 'BUT' named entity 'EPIDEMIC' named entity 'IDENTIFICATION' named entity 'RESPIRATORY INFECTIONS' named entity 'SPECIMENS' named entity 'RESPIRATORY INFECTION' named entity 'MATCHED' named entity 'CONSEQUENCE' named entity 'VIRAL PATHOGENS'

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