About: RNA elements required for RNA recombination function as replication enhancers in vitro and in vivo in a plus‐strand RNA virus

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About: RNA elements required for RNA recombination function as replication enhancers in vitro and in vivo in a plus‐strand RNA virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	RNA elements required for RNA recombination function as replication enhancers in vitro and in vivo in a plus‐strand RNA virus
Creator	Pogany, Judit Nagy, Peter Simon, Anne
Source	Medline; PMC
abstract	RNA replication requires cis‐acting elements to recruit the viral RNA‐dependent RNA polymerase (RdRp) and facilitate de novo initiation of complementary strand synthesis. Hairpins that are hot spots for recombination in the genomic RNA of turnip crinkle virus (TCV) and satellite (sat)‐RNA C, a parasitic RNA associated with TCV infections, stimulate RNA synthesis 10‐fold from a downstream promoter sequence in an in vitro assay using partially purified TCV RdRp. Artificial hairpins had an inhibitory effect on transcription. RNA accumulation in single cells was enhanced 5‐ to 10‐fold when the natural stem‐loop structures were inserted into a poorly accumulating sat‐RNA. The effect of the stem‐loop structures on RNA replication was additive, with insertion of three stem‐loop RNA elements increasing sat‐RNA accumulation to the greatest extent (25‐fold). These stem‐loop structures do not influence the stability of the RNAs in vivo, but may serve to recruit the RdRp to the template.
has issue date	1999-10-15(xsd:dateTime)
bibo:doi	10.1093/emboj/18.20.5653
bibo:pmid	10523308
has license	no-cc
sha1sum (hex)	1f06fdb16fef50aa58a274874dec6266c7f206e8
schema:url	https://doi.org/10.1093/emboj/18.20.5653
resource representing a document's title	RNA elements required for RNA recombination function as replication enhancers in vitro and in vivo in a plus‐strand RNA virus
has PubMed Central identifier	PMC1171632
has PubMed identifier	10523308
schema:publication	EMBO J
resource representing a document's body	covid:1f06fdb16fef50aa58a274874dec6266c7f206e8#body_text
is schema:about of	named entity 'RNA' named entity 'stimulate' named entity 'sat' named entity 'RNA' named entity 'elements' named entity 'PARASITIC' named entity 'IN VITRO ASSAY' named entity 'RNA REPLICATION' covid:arg/1f06fdb16fef50aa58a274874dec6266c7f206e8 named entity 'de novo' named entity 'influence' named entity 'satellite' named entity 'natural' named entity 'elements' named entity 'structures' named entity 'RNA' named entity 'single' named entity 'stem-loop structures' named entity 'RNAs' named entity 'RNA elements' named entity 'turnip crinkle virus' named entity 'TCV' named entity 'RNA' named entity 'RNA replication' named entity 'stem-loop structures' named entity 'RNA' named entity 'RNA virus' named entity 'protoplasts' named entity 'RNAs' named entity 'trans-activator' named entity 'enhancer' named entity 'protoplast' named entity 'RNA' named entity 'protoplasts' named entity 'RNAs' named entity 'ethidium bromide' named entity 'RNA' named entity 'RNA' named entity 'non-coding region' named entity 'RNA' named entity 'RNA replication' named entity 'protoplasts' named entity 'turnip' named entity 'transcription enhancers' named entity 'PCR' named entity 'RNA' named entity 'transactivation' named entity 'citrus tristeza virus' named entity 'RNA' named entity 'symptom' named entity 'RNA' named entity 'replicase' named entity 'protoplasts' named entity 'RNA' named entity 'dimers' named entity 'modular evolution' named entity 'minus-strand' named entity 'Beck' named entity 'virus evolution' named entity 'Monomeric' named entity 'RdRp' named entity 'RNA' named entity 'RNAs' named entity 'RNA replication' named entity 'cis' named entity 'RNA' named entity 'RNA' named entity 'cis' named entity 'RdRp' named entity 'transcription initiation' named entity 'PCR-based' named entity 'flock house virus' named entity 'TCV' named entity 'riboprobe' named entity 'RNA' named entity 'RNA' named entity 'complementary strand' named entity 'RNA replication' named entity 'RNA'

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