About: RNA replication requires cis‐acting elements to recruit the viral RNA‐dependent RNA polymerase (RdRp) and facilitate de novo initiation of complementary strand synthesis. Hairpins that are hot spots for recombination in the genomic RNA of turnip crinkle virus (TCV) and satellite (sat)‐RNA C, a parasitic RNA associated with TCV infections, stimulate RNA synthesis 10‐fold from a downstream promoter sequence in an in vitro assay using partially purified TCV RdRp. Artificial hairpins had an inhibitory effect on transcription. RNA accumulation in single cells was enhanced 5‐ to 10‐fold when the natural stem‐loop structures were inserted into a poorly accumulating sat‐RNA. The effect of the stem‐loop structures on RNA replication was additive, with insertion of three stem‐loop RNA elements increasing sat‐RNA accumulation to the greatest extent (25‐fold). These stem‐loop structures do not influence the stability of the RNAs in vivo, but may serve to recruit the RdRp to the template.   Goto Sponge  NotDistinct  Permalink

An Entity of Type : fabio:Abstract, within Data Space : covidontheweb.inria.fr associated with source document(s)

AttributesValues
type
value
  • RNA replication requires cis‐acting elements to recruit the viral RNA‐dependent RNA polymerase (RdRp) and facilitate de novo initiation of complementary strand synthesis. Hairpins that are hot spots for recombination in the genomic RNA of turnip crinkle virus (TCV) and satellite (sat)‐RNA C, a parasitic RNA associated with TCV infections, stimulate RNA synthesis 10‐fold from a downstream promoter sequence in an in vitro assay using partially purified TCV RdRp. Artificial hairpins had an inhibitory effect on transcription. RNA accumulation in single cells was enhanced 5‐ to 10‐fold when the natural stem‐loop structures were inserted into a poorly accumulating sat‐RNA. The effect of the stem‐loop structures on RNA replication was additive, with insertion of three stem‐loop RNA elements increasing sat‐RNA accumulation to the greatest extent (25‐fold). These stem‐loop structures do not influence the stability of the RNAs in vivo, but may serve to recruit the RdRp to the template.
subject
  • Biotechnology
  • RNA
  • Gene expression
  • DNA
  • Nucleic acids
  • Senescence
  • RNA splicing
  • EC 2.7.7
  • Molecular biology
  • Viral plant pathogens and diseases
part of
is abstract of
is hasSource of
Faceted Search & Find service v1.13.91 as of Mar 24 2020


Alternative Linked Data Documents: Sponger | ODE     Content Formats:       RDF       ODATA       Microdata      About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data]
OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software