About: chromium release assay

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An Entity of Type : owl:Class, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Class
label	chromium release assay
subClassOf	in vitro cell killing assay nodeID://b665208281 nodeID://b665208290 nodeID://b665232578 nodeID://b665232587 nodeID://b665831100 nodeID://b665831109 nodeID://b665855397 nodeID://b665855406 nodeID://b665887612 nodeID://b665887621 nodeID://b665911909 nodeID://b665911918 nodeID://b665936206 nodeID://b665936215 nodeID://b665960503 nodeID://b665960512 nodeID://b669096076 nodeID://b669096085 nodeID://b669120261 nodeID://b669120270
described by	proxy:entity/http/purl.obolibrary.org/obo/obi.owl
term editor	IEDB PlanAndPlannedProcess Branch
example of usage	Autologous EBV-transformed B-LCL were used as target cells for the influenza virus-specific CTL assays. Equal volumes of target and effector cells were added to triplicate wells of 96-well tissue culture plates, and 1:2 serial dilutions of effectors were made, producing effector-to-target (E:T) ratios of 100:1, 50:1, 25:1, and 12.5:1. After a 4-h incubation of the effector cells with the target cells, supernatants were collected following brief centrifugation and transferred to polystyrene tubes to be counted with the LKB 1272 Clinigamma counter (Wallac). Percent specific killing was determined with the following equation: (experimental 51Cr release - spontaneous 51Cr release)/(maximum 51Cr release - spontaneous 51Cr release) x 100.
definition	An in vitro cell killing assay in which radioactive chromium is absorbed by cells and released into supernatant when the cells die. The amount of radioactivity measured in the supernatant is a proxy for the number of cells that have died. An in vitro cell killing assay in which radioactive chromium is absorbed by cells and released into supernatant when the cells die. The amount of radioactivity measured in the supernatant is a proxy for the number of cells that have died.
editor preferred term	chromium release assay
has curation status	metadata incomplete
is first of	nodeID://b665200149 nodeID://b665224446 nodeID://b665822968 nodeID://b665847265 nodeID://b665879480 nodeID://b665903777 nodeID://b665928074 nodeID://b665952371 nodeID://b669088010 nodeID://b669112195
is subClassOf of	51 chromium assay measuring epitope specific T cell killing
is topic of	Ontology for Biomedical Investigations

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