About: enzyme-linked immunospot assay   Goto Sponge  NotDistinct  Permalink

An Entity of Type : owl:Class, within Data Space : covidontheweb.inria.fr associated with source document(s)

AttributesValues
type
label
  • enzyme-linked immunospot assay
subClassOf
described by
term editor
  • IEDB
example of usage
  • Determination of the frequency of cells producing IFN-gamma in response to viral peptides by placing effector cells on a anti IFN-gamma coated plate, and adding antigen presenting cells pulsed with a peptide. After washing, the bound IFN-gamma is detected with a secondary antibody linked to a dye that visualizes as one spot per cell.
definition
  • A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest.
  • A cytometry assay in which cells are cultured on a surface coated with a capture antibody binding a secretory material of interest which subsequently gets stained resulting in a spot for each cell producing the secretory material of interest.
definition source
  • IEDB
alternative label
  • ELISPOT assay
editor preferred term
  • enzyme-linked immunospot assay
has curation status
is first of
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