About: transcript expression location detection by hybridization chain reaction assay   Goto Sponge  NotDistinct  Permalink

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label
  • transcript expression location detection by hybridization chain reaction assay
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term tracker item
  • https://github.com/obi-ontology/obi/issues/783
term editor
  • Paul D. Thomas
  • Yang Chai
example of usage
  • PMID 25977364: "Multiplexed imaging of mRNA expression using fluorescent hybridization chain reaction (HCR) quantitatively confirmed the expression profiles of lead cells"
definition
  • An in situ hybridization assay in which the location (e.g. anatomical/tissue) of a transcript is detected by a multiplexed fluorescent in situ hybridization, based on orthogonal amplification with hybridization chain reactions (HCR). RNA probes complementary to mRNA targets trigger chain reactions in which fluorophore-labeled RNA hairpins self-assemble into tethered fluorescent amplification polymers.
  • An in situ hybridization assay in which the location (e.g. anatomical/tissue) of a transcript is detected by a multiplexed fluorescent in situ hybridization, based on orthogonal amplification with hybridization chain reactions (HCR). RNA probes complementary to mRNA targets trigger chain reactions in which fluorophore-labeled RNA hairpins self-assemble into tethered fluorescent amplification polymers.
definition source
  • PMID:21037591
alternative label
  • transcript expression location detection by fluorescent HCR.
editor preferred term
  • transcript expression location detection by hybridization chain reaction assay
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