The neurotransmitter was assessed by co-labelling cells with a Cha-GAL4.7.4 driver [FBtp0014830] and an anti-ChAT antibody (Varija Raghu et al., 2011). Pre- versus postsynaptic innervation judged by scoring of terminal morphology from figures in Fischbach and Dittrich (1989) as assessed by FlyBrain Neuron DB.
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