About: Development of A Loop-Mediated Isothermal Amplification (LAMP) for the Detection of F5 Fimbriae Gene in Enterotoxigenic Escherichia coli (ETEC)

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About: Development of A Loop-Mediated Isothermal Amplification (LAMP) for the Detection of F5 Fimbriae Gene in Enterotoxigenic Escherichia coli (ETEC) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of A Loop-Mediated Isothermal Amplification (LAMP) for the Detection of F5 Fimbriae Gene in Enterotoxigenic Escherichia coli (ETEC)
Creator	Zhu, Ying Shi, Dongfang Liu, Wenxin Feng, Yufei Guan, Weikun He, Lili Hu, Wenxia Jiang, Kuiyu
Source	Medline; PMC
abstract	The objective of this study was to establish a loop-mediated isothermal amplification (LAMP) method for the detection of F5 fimbriae gene in Enterotoxigenic Escherichia coli. A set of four primers were designed based on the conservative sequence of coding F5 fimbriae. Temperature and time condition, specificity test, and sensitivity test were performed with the DNA of Escherichia coli (F5+). The results showed that the optimal reaction condition for LAMP was achieved at 61 °C for 45 min in a water bath. Ladder-like products were produced with those F5-positive samples by LAMP, while no product was generated with other negative samples. The assay of LAMP had a detection limit equivalent to 72 cfu/tube, which was more sensitive than PCR (7.2 × 10(2) cfu/tube). The agreement rate between LAMP and PCR was 100 % in detecting simulation samples. Thus, the LAMP assay may be a new method for rapid detection of F5 fimbriae gene of ETEC.
has issue date	2012-08-14(xsd:dateTime)
bibo:doi	10.1007/s00284-012-0204-6
bibo:pmid	22890294
has license	no-cc
sha1sum (hex)	fff60988428b82658458d381a6c27583209a686e
schema:url	https://doi.org/10.1007/s00284-012-0204-6
resource representing a document's title	Development of A Loop-Mediated Isothermal Amplification (LAMP) for the Detection of F5 Fimbriae Gene in Enterotoxigenic Escherichia coli (ETEC)
has PubMed Central identifier	PMC7080040
has PubMed identifier	22890294
schema:publication	Curr Microbiol
resource representing a document's body	covid:fff60988428b82658458d381a6c27583209a686e#body_text
is schema:about of	named entity 'FIMBRIAE' named entity 'samples' named entity 'reaction' named entity 'RAPID' named entity 'NEGATIVE' named entity 'MIN' named entity '100' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'ASSAY' named entity 'NEW' named entity 'SENSITIVE' named entity 'SENSITIVITY TEST' named entity 'TIME' named entity 'DETECTION LIMIT' named entity 'GENE' named entity 'SEQUENCE' named entity 'STUDY' named entity 'PRODUCTS' named entity 'REACTION CONDITION' named entity 'POSITIVE' named entity 'TEMPERATURE' named entity 'RATE' named entity 'MAY BE A' named entity 'DNA' named entity 'OBJECTIVE' named entity 'PRODUCT' named entity 'AGREEMENT' named entity 'SIMULATION' named entity 'ESCHERICHIA COLI' named entity 'PCR' named entity 'METHOD' named entity 'WATER BATH' named entity 'DETECTING' named entity 'LIKE' named entity 'GENE' named entity 'ENTEROTOXIGENIC ESCHERICHIA COLI' named entity 'SAMPLES' named entity 'TEST' named entity 'OPTIMAL' named entity 'CFU' named entity 'FIMBRIAE' named entity 'ENTEROTOXIGENIC ESCHERICHIA COLI' named entity 'PERFORMED' named entity 'PRIMERS' named entity 'LADDER' named entity 'DETECTION' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'DEVELOPMENT' named entity 'SPECIFICITY' named entity 'DETECTION' named entity 'AT 61' named entity 'BASED' named entity 'GENERATED' named entity 'RESULTS' named entity 'LAMP' named entity '287' named entity 'SET' named entity 'CONDITION' named entity 'ESTABLISH' named entity 'CODING' named entity 'EQUIVALENT' covid:arg/fff60988428b82658458d381a6c27583209a686e named entity 'cfu' named entity 'sequence' named entity 'study' named entity 'min' named entity 'LAMP' named entity 'Escherichia coli' named entity 'Enterotoxigenic Escherichia coli' named entity 'Detection' named entity 'Amplification' named entity 'PCR' named entity '7.2' named entity 'ETEC'

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