About: Evaluation of swine enteroids as in vitro models for Lawsonia intracellularis infection(,)

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of swine enteroids as in vitro models for Lawsonia intracellularis infection(,)
Creator	Gebhart, Connie Resende, Talita Vannucci, Fabio Saqui-Salces, Milena Medida, Ramya Lund, Lacey Vasquez, Erika
Source	Medline; PMC
abstract	The enteric pathogen Lawsonia intracellularis is one of the main causes of diarrhea and compromised weight gain in pigs worldwide. Traditional cell-line cultures have been used to study L. intracellularis pathogenesis. However, these systems fail to reproduce the epithelial changes observed in the intestines of L. intracellularis-infected pigs, specifically, the changes in intestinal cell constitution and gene expression. A more physiologically accurate and state-of-the-art model is provided by swine enteroids derived from stem cell-containing crypts from healthy pigs. The objective of this study was to verify the feasibility of two-dimensional swine enteroids as in vitro models for L. intracellularis infection. We established both three- and two-dimensional swine enteroid cultures derived from intestinal crypts. The two-dimensional swine enteroids were infected by L. intracellularis in four independent experiments. Enteroid-infected samples were collected 3 and 7 d postinfection for analysis using real-time quantitative PCR and L. intracellularis immunohistochemistry. In this study, we show that L. intracellularis is capable of infecting and replicating intracellularly in two-dimensional swine enteroids derived from ileum.
has issue date	2020-01-16(xsd:dateTime)
bibo:doi	10.1093/jas/skaa011
bibo:pmid	31943029
has license	no-cc
sha1sum (hex)	fe86a5bd673bcbd1fa38f1598671263a75e2cbdc
schema:url	https://doi.org/10.1093/jas/skaa011
resource representing a document's title	Evaluation of swine enteroids as in vitro models for Lawsonia intracellularis infection(,)
has PubMed Central identifier	PMC7007770
has PubMed identifier	31943029
schema:publication	J Anim Sci
resource representing a document's body	covid:fe86a5bd673bcbd1fa38f1598671263a75e2cbdc#body_text
is schema:about of	named entity 'ORCID' covid:arg/fe86a5bd673bcbd1fa38f1598671263a75e2cbdc named entity 'LAWSONIA INTRACELLULARIS' named entity 'SWINE' named entity 'NUMBERS' named entity 'MODELS' named entity 'EVALUATION' named entity 'INFECTION' named entity 'IN VITRO' named entity 'ORCiD' named entity 'Resende' named entity 'Lawsonia intracellularis' named entity 'swine' named entity 'aspirating' named entity 'swine' named entity 'Hanks' Balanced Salt solution' named entity 'mice' named entity 'intestinal epithelial cell' named entity 'intestinal epithelial cells' named entity 'antigen' named entity 'infection' named entity 'centrifuged' named entity 'infection' named entity 'real-time PCR' named entity 'epithelial cell' named entity 'signaling pathways' named entity 'gene editing' named entity 'syringe' named entity 'PCR' named entity 'penicillin' named entity 'vaccines' named entity 'antigen' named entity 'prevention and control' named entity 'intestinal epithelial' named entity 'bacteria' named entity 'pig' named entity 'infection' named entity 'ATCC' named entity 'intestine' named entity 'EDTA' named entity 'fibroblast cells' named entity 'DMEM' named entity 'intestinal stem cells' named entity 'PCR' named entity 'microcentrifuge' named entity 'phosphate-buffered saline' named entity 'euthanasia' named entity 'immunohistochemistry' named entity 'crypt hyperplasia' named entity 'Swine' named entity 'intestine' named entity 'intestinal epithelium' named entity 'host cells' named entity 'genetic modification' named entity 'supernatant' named entity 'streptomycin' named entity 'genus' named entity 'infection' named entity 'infection' named entity 'laboratory conditions' named entity 'swine' named entity 'endocytic' named entity 'real-time PCR' named entity 'swine' named entity 'biopsy' named entity 'antimicrobial' named entity 'microinjection' named entity 'magnesium' named entity 'host-pathogen interactions' named entity 'swine' named entity 'PBS' named entity 'DMEM' named entity 'Matrigel' named entity 'infection' named entity 'Matrigel'

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