About: MicroRNA-based strategy to mitigate the risk of gain-of-function influenza studies

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	MicroRNA-based strategy to mitigate the risk of gain-of-function influenza studies
Creator	Angel, Matthew Sutton, Troy Xu, Kemin García-Sastre, Adolfo Perez, Daniel Finch, Courtney Kimble, Brian Chua, Mark Langlois #1, Ryan Shapiro, Jillian Silvia Gonzalez-Reiche, Ana Tenoever, Benjamin
Source	PMC
abstract	Recent gain-of-function studies in influenza A virus H5N1 strains revealed that as few as three amino-acid changes in the hemagglutinin protein confer the capacity for viral transmission between ferrets(1, 2). As transmission between ferrets is considered a surrogate indicator of transmissibility between humans, these studies raised concerns about the risks of gain-of-function influenza A virus research. Here we present an approach to strengthen the biosafety of gain-of-function influenza experiments. We exploit species-specific endogenous small RNAs to restrict influenza A virus tropism. In particular, we found that the microRNA miR-192 was expressed in primary human respiratory tract epithelial cells as well as mouse lungs but absent from the ferret respiratory tract. Incorporation of miR-192 target sites into influenza A virus did not prevent influenza replication and transmissibility in ferrets, but did attenuate influenza pathogenicity in mice. This molecular biocontainment approach should be applicable beyond influenza A virus to minimize the risk of experiments involving other pathogenic viruses.
has issue date	2013-08-11(xsd:dateTime)
bibo:doi	10.1038/nbt.2666
bibo:pmid	23934176
has license	no-cc
sha1sum (hex)	fdc336253485b3b3e895271a27f3457611e826f4
schema:url	https://doi.org/10.1038/nbt.2666
resource representing a document's title	MicroRNA-based strategy to mitigate the risk of gain-of-function influenza studies
has PubMed Central identifier	PMC3808852
has PubMed identifier	23934176
schema:publication	Nat Biotechnol
resource representing a document's body	covid:fdc336253485b3b3e895271a27f3457611e826f4#body_text
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