About: Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla

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About: Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla
Creator	Chen, Bin Ge, Junqing Yang, Jinxian Gong, Hui Chi, Hongshu Fang, Qinmei Zheng, Zaiyu
Source	PMC
abstract	In the present study, a new hepatic tissue‐origin cell line from European eel Anguilla anguilla has been developed and characterized. This cell line designated EL has been maintained in Leibovitz L‐15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL cell line consisted predominantly of fibroblast‐like cells, which could survive over 100 days in vitro, and could grow at 15–32°C. The optimum temperature for growth was 27°C. The chromosome analysis revealed a modal diploid karyotype of 2n = 38. The origin of this cell line was confirmed by the 18S recombinant (r)RNA sequencing. The susceptibility test indicated significant cytopathic effects in the EL cells with regard to the Rana grylio virus and the Herpesvirus anguillae. The viral replication was confirmed by transmission electron microscopy and polymerase chain reaction analysis. Following poly (I:C) exposure, the expression levels of the immune‐related molecules interferon regulatory factor‐7 (irf7) and transforming growth factor‐β (TGF‐β) were downregulated in EL cells, whereas the expression levels of the rf3 and the cytochrome P450 (CYP450) were upregulated. All four genes were significantly upregulated following inflammation by lipopolysaccharide (LPS). These data suggested the application of EL cell line for viral identification, as well as for immunodiagnosis and pharmacological targeting.
has issue date	2019-12-19(xsd:dateTime)
bibo:doi	10.1002/cbin.11276
bibo:pmid	31814207
has license	cc-by
sha1sum (hex)	fb8e2e76092de55f4b7f22bd0106f6c3853ca3bd
schema:url	https://doi.org/10.1002/cbin.11276
resource representing a document's title	Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla
has PubMed Central identifier	PMC7028054
has PubMed identifier	31814207
schema:publication	Cell Biol Int
resource representing a document's body	covid:fb8e2e76092de55f4b7f22bd0106f6c3853ca3bd#body_text
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