About: A novel multiplex PCR for virus detection by melting curve analysis

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A novel multiplex PCR for virus detection by melting curve analysis
Creator	Xu, Jin Jia, Ran Cao, Lingfeng Dong, Niuniu Dong, Zuoquan Liu, Pengcheng Lu, Lijuan Su, Liyun Xu, Menghua Zhong, Huaqing Zhou, Linfu
Source	Elsevier; Medline; PMC
abstract	Abstract Background Rapid and accurate laboratory diagnoses of viral infections are crucial for the management and treatment of patients with viral infections. Conventional methods for virus detection are labourious, time consuming, and only a single virus can be analysed in one assay. Objectives The objective of this study was to develop a novel real-time PCR method for multiple virus detection by melting curve analysis using Taqman probes in a single reaction. Study design As a model, six respiratory viruses were detected in one tube using three fluorophores. The specificity was assessed by cross-reaction tests with other common respiratory pathogens. The analytical sensitivity was assessed by testing the limit of detection of the assay using artificial plasmids as the positive template. The clinical evaluation of the established assay was evaluated for the detection of respiratory viruses in clinical samples, and the results were compared with direct fluorescent antibody testing (DFA). Results The six respiratory viruses were clearly distinguished by their respective melting temperature values in the corresponding fluorescence detection channels. No cross reactions were observed by cross reaction tests. The detection limits of this assay were 2 to 2 × 103 copies per reaction for each virus. The clinical evaluation of this assay was demonstrated by analysing 352 clinical samples, and 67(19.0%) samples were positive for at least one virus. The accordance rate between the established PCR and DFA testing was high, and ranged from 94.57% to 100%. Conclusions Taqman probe-based melting curve analysis is well suited for detection of multiple viruses in clinical and research laboratories because of its high throughput, reliability, and cost savings.
has issue date	2018-12-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2018.09.010
bibo:pmid	30267725
has license	els-covid
sha1sum (hex)	fb1727cba632fd315e7d816852f48456c60fd8df
schema:url	https://doi.org/10.1016/j.jviromet.2018.09.010
resource representing a document's title	A novel multiplex PCR for virus detection by melting curve analysis
has PubMed Central identifier	PMC7113878
has PubMed identifier	30267725
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:fb1727cba632fd315e7d816852f48456c60fd8df#body_text
is schema:about of	named entity 'fluorescence' named entity 'fluorescent antibody testing' named entity 'positive' named entity 'tests' named entity 'assessed' named entity 'melting temperature' named entity 'respiratory' named entity 'PCR' named entity 'evaluation' named entity 'tests' named entity 'viral infections' named entity 'viruses' named entity 'NOVEL' named entity 'NOVEL' named entity 'FLUORESCENT ANTIBODY' named entity 'POSITIVE' named entity 'HIGH' named entity 'METHODS' named entity 'RESULTS' named entity 'DETECTION' named entity 'CROSS REACTION' named entity 'CROSS-REACTION' named entity 'THEIR' named entity 'RESPIRATORY PATHOGENS' named entity 'ASSAY' named entity 'SINGLE' named entity 'CONSUMING' named entity 'VIRAL INFECTIONS' named entity 'ACCURATE' named entity 'CROSS REACTIONS' named entity 'CHANNELS' named entity 'COMMON' named entity 'DETECTED' named entity 'LABORATORY' named entity 'DIAGNOSES' named entity 'VIRUS DETECTION' named entity 'RAPID' named entity 'HIGH THROUGHPUT' named entity 'ANTIBODY TESTING' named entity 'CLINICAL EVALUATION' named entity 'DFA' named entity 'SENSITIVITY' named entity 'USING' named entity '100%' named entity 'LIMITS' named entity 'REACTION' named entity 'DIRECT' named entity 'MODEL' named entity 'TEMPLATE' covid:arg/fb1727cba632fd315e7d816852f48456c60fd8df named entity 'CONCLUSIONS' named entity 'RESPIRATORY VIRUSES' named entity 'COMPARED' named entity 'REAL-TIME PCR ' named entity 'TIME' named entity 'MELTING CURVE ANALYSIS' named entity 'RESEARCH LABORATORIES' named entity 'VIRUSES' named entity 'EVALUATED' named entity 'ESTABLISHED' named entity 'COST SAVINGS' named entity 'BASED' named entity 'COPIES' named entity 'FLUORESCENCE DETECTION' named entity 'TUBE' named entity 'TAQMAN' named entity '352' named entity 'CLINICAL' named entity 'TREATMENT' named entity 'MELTING CURVE ANALYSIS' named entity 'VIRUS DETECTION'

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