About: MG132 Attenuates the Replication of Classical Swine Fever Virus in vitro

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About: MG132 Attenuates the Replication of Classical Swine Fever Virus in vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	MG132 Attenuates the Replication of Classical Swine Fever Virus in vitro
Creator	Zhou, Bin Zhang, Jingyuan Chen, Jinding Zou, Wei Ding, Hongxing Fan, Shuangqi Ma, Shengming Wu, Keke Yi, Lin Zhao, Mingqiu Zhu, Erpeng Chen, Yuming Deng, Shaofeng He, Wencheng Xu, Hailuan Zhao, Mengpo
source	Medline; PMC
abstract	The 26S proteasome, in charge of intracellular protein degradation, plays significant roles in the modulation of various cellular activities as well as in the interplay between virus and host. However, studies about the relationship between 26S proteasome and classical swine fever virus (CSFV) is limited up to now. MG132 is a proteasome inhibitor and has been extensively used in studies about replication of many viruses. Herein, we investigated the role of MG132 in CSFV replication and results showed that MG132 significantly decreased virus titers and viral RNA copies in CSFV-infected PK-15 cells. Further studies demonstrated that MG132 upregulated the expression of several interferon-stimulated genes (ISGs), in CSFV-infected cells. Since the activation of ISGs is controlled by the JAK-STAT signal pathway, we next examined the effect of MG132 on the expression and localization of key molecular STAT1 in the infected cells using Western blot and confocal laser scanning microscopy, respectively. Results showed that CSFV infection and viral NS4A protein decreased the protein level of STAT1, and MG132 promoted the accumulation of STAT1 in the nucleus of cells adjacent to the CSFV-infected cells. Besides, MG132 did not affect the expressions of IFN-α, STAT1, Mx1, OAS1, and PKR genes in cells without CSFV. In conclusion, we identify that MG132 significantly inhibits CSFV replication in vitro, in which the activation of the JAK-STAT pathway and the subsequent upregulation of expressions of ISGs might play significant roles, providing a potential preventive method for CSF.
has issue date	2020-06-03(xsd:dateTime)
bibo:doi	10.3389/fmicb.2020.00852
bibo:pmid	32582037
has license	cc-by
sha1sum (hex)	fa6b370189773c2e4249a05d9e3531d2cca83905
schema:url	https://doi.org/10.3389/fmicb.2020.00852
resource representing a document's title	MG132 Attenuates the Replication of Classical Swine Fever Virus in vitro
has PubMed Central identifier	PMC7283581
has PubMed identifier	32582037
schema:publication	Front Microbiol
resource representing a document's body	covid:fa6b370189773c2e4249a05d9e3531d2cca83905#body_text
is schema:about of	named entity 'EXPRESSION' named entity 'RESULTS' named entity 'PROTEIN DEGRADATION' named entity 'CONFOCAL LASER SCANNING MICROSCOPY' named entity 'IN VITRO' named entity 'EFFECT' named entity 'ROLE' named entity 'KEY' named entity 'MOLECULAR' named entity 'IFN' named entity 'RELATIONSHIP' named entity 'PK-' named entity 'CHARGE' named entity 'NS4A' named entity 'VARIOUS' named entity 'ADJACENT TO' named entity 'NUCLEUS' named entity 'INTRACELLULAR' named entity 'CSF' named entity 'MG132' named entity 'intracellular' named entity 'JAK-STAT' named entity 'protein' named entity 'viral RNA' named entity 'signal pathway' named entity 'JAK-STAT pathway' named entity 'classical swine fever virus' named entity 'MG132' named entity 'CSFV' named entity 'CSFV' named entity 'classical swine fever virus' named entity 'MG132' named entity 'Classical Swine Fever Virus' named entity 'IFN' named entity 'Clontech' named entity 'Mx1' named entity 'DMSO' named entity 'DMSO' named entity 'antiviral' named entity '26S proteasome' named entity 'porcine' named entity 'primary antibodies' named entity 'ISGs' named entity 'protein' named entity '26S proteasome' named entity 'MG132' named entity 'viral proteins' named entity 'MG132' named entity 'qRT-PCR' named entity 'CSFV' named entity 'primary antibodies' named entity 'IFN' named entity 'upregulation' named entity 'CSFV' named entity 'antibody' named entity 'upregulation' named entity 'STAT1' named entity 'endogenous' named entity 'TRITC' named entity 'antiviral' named entity 'Western blot' named entity 'MG132' named entity 'Upregulation' named entity 'absolute ethanol' named entity 'ISGs' named entity 'ubiquitin'

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