About: High-Throughput Transcription-mediated amplification on the Hologic Panther is a highly sensitive method of detection for SARS-CoV-2

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About: High-Throughput Transcription-mediated amplification on the Hologic Panther is a highly sensitive method of detection for SARS-CoV-2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	High-Throughput Transcription-mediated amplification on the Hologic Panther is a highly sensitive method of detection for SARS-CoV-2
Creator	Verma, Subhash Gorzalski, Andrew Laverdure, Chris Morzunov, Sergey Pandori, Mark Tian, Honglin Vanhooser, Stephanie
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: As the demand for laboratory testing for SARS-CoV-2 increases, additional varieties of testing methodologies are being considered. While real time polymerase chain reaction (RT-PCR) has performed as the main method for virus detection, other methods are becoming available, including transcription mediated amplification (TMA). The Hologic Aptima SARS-CoV-2 Assay utilizes TMA as a target amplification mechanism, and it has only recently received Emergency Use Authorization (EUA) by the Food and Drug Administration (FDA). OBJECTIVES: We sought to compare the sensitivity and specificity of the Aptima SARS-CoV-2 Assay to RTPCR as a means of SARS-CoV-2 detection in a diagnostic setting. STUDY DESIGN: We performed a limit-of-detection study (LoD) to assess the analytical sensitivity of TMA and RT-PCR. This preceded a comparison of the methods using previously evaluated clinical specimens (nasopharyngeal swabs) using 116 human specimens tested by both methodologies. Specimens included sixty-one (61) specimens found reactive by real-time PCR, fifty-one (51) found non-reactive, and four (4) deemed inconclusive. RESULTS: The Aptima SARS-CoV-2 Assay showed a markedly higher analytical sensitivity than RT-PCR by LoD study. Evaluation of clinical specimens resulted in fewer inconclusive results by the SARS-CoV-2 assay, leading to potentially higher clinical sensitivity. CONCLUSIONS: The higher analytical sensitivity may explain the assay’s ability to ascertain for the presence of SARS-CoV-2 genome in human specimens deemed inconclusive by real-time PCR. TMA provides an effective, highly sensitive means of detection of SARS-CoV-2 in nasopharyngeal specimens.
has issue date	2020-06-10(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2020.104501
bibo:pmid	32619959
has license	no-cc
sha1sum (hex)	f9d899add666be69dec68296efdef3dadf6d425c
schema:url	https://doi.org/10.1016/j.jcv.2020.104501
resource representing a document's title	High-Throughput Transcription-mediated amplification on the Hologic Panther is a highly sensitive method of detection for SARS-CoV-2
has PubMed Central identifier	PMC7286273
has PubMed identifier	32619959
schema:publication	J Clin Virol
resource representing a document's body	covid:f9d899add666be69dec68296efdef3dadf6d425c#body_text
is schema:about of	named entity 'highly' named entity 'detection' named entity 'Hologic' named entity 'SARS-CoV-2' named entity 'primer' named entity 'RT-PCR' named entity 'Hologic' named entity 'viral genome' named entity 'Hologic' named entity 'RT-PCR' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'viral genomes' named entity 'Hologic' named entity 'RT-PCR' named entity 'COVID-19 pandemic' named entity 'VTM' named entity 'Hologic' named entity 'SARS-CoV-2' named entity 'TMA' named entity 'Manassas, VA' named entity 'Hologic' named entity 'transcription mediated amplification' named entity 'TMA' named entity 'lysis buffer' named entity 'RT-PCR' named entity 'PCR' named entity 'clinical laboratories' named entity 'public health' named entity 'TMA' named entity 'RT-PCR' named entity 'public health' named entity 'public health laboratories' named entity 'nasopharyngeal swabs' named entity 'TMA' named entity 'Hologic' named entity 'lysed' named entity 'TMA' named entity 'virus' named entity 'SARS-CoV-2' named entity 'RT-PCR' named entity 'false negative results' named entity 'SARS-CoV-2' named entity 'nasopharyngeal swabs' named entity 'diagnostic testing' named entity 'FDA' named entity 'genomes' named entity 'Food and Drug Administration' named entity 'PCR' named entity 'TMA' named entity 'real time PCR' named entity 'pathology' named entity 'RT-PCR' named entity 'TMA' named entity 'TMA' named entity 'RT-PCR' named entity 'RT-PCR' named entity 'TMA' named entity '2019-nCoV' named entity 'VTM' named entity 'transcriptional' named entity 'real-time PCR' named entity 'SARS-CoV-2' named entity 'scientific community' named entity 'COVID' named entity 'lysis' named entity 'nucleic acid' named entity 'CDC' named entity 'high-throughput' named entity 'real time PCR' named entity 'high-throughput' named entity 'SARS-CoV-2' named entity 'Hologic' named entity 'gene' named entity 'Hologic'

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