About: In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene

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About: In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene
Creator	Wang, Jing Wang, Gang He, Lei Cheng, Min Dong, Ling-Juan Tang, Qing-Hai Zhang, Yan-Ming
Source	Medline; PMC
abstract	BACKGROUND: Transmissible gastroenteritis (TGE) is a highly contagious viral disease of swine, characterized by severe vomiting, diarrhea, and high mortality. Currently, the vaccines for it are only partially effective and no specific drug is available for treatment of TGE virus (TGEV) infection. RNA interference has been confirmed as a new approach for controlling viral infections. In this study, the inhibitory effect of short hairpin RNAs (shRNAs) targeting the ORF 7 gene of TGEV on virus replication was examined. RESULTS: Four theoretically effective sequences of TGEV ORF 7 gene were designed and selected for construction of shRNA expression plasmids. In the reporter assays, three of four shRNA expression plasmids were able to inhibit significantly the expression of ORF 7 gene and replication of TGEV, as shown by real-time quantitative RT-PCR analysis of viral ORF 7 and N genes and detection of virus titers (TCID(50)/ml). Stable swine testicular (ST) cells expressing the shRNAs were established. Observation of the cytopathic effect and apoptosis, as well as a cell proliferation assay demonstrated that the three shRNAs were capable of protecting ST cells against TGEV destruction, with high specificity and efficiency. CONCLUSIONS: Our results indicated that plasmid-transcribed shRNAs targeting the ORF 7 gene in the TGEV genome effectively inhibited expression of the viral target gene and viral replication in vitro. These findings provide evidence that the shRNAs have potential therapeutic application for treatment of TGE.
has issue date	2012-08-28(xsd:dateTime)
bibo:doi	10.1186/1743-422x-9-176
bibo:pmid	22929207
has license	cc-by
sha1sum (hex)	f9c39fa24056184c67ec47da4da93ba583c36e84
schema:url	https://doi.org/10.1186/1743-422x-9-176
resource representing a document's title	In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene
has PubMed Central identifier	PMC3492083
has PubMed identifier	22929207
schema:publication	Virol J
resource representing a document's body	covid:f9c39fa24056184c67ec47da4da93ba583c36e84#body_text
is schema:about of	named entity 'cells' named entity 'vomiting' named entity 'ORF' named entity 'virus' named entity 'ORF' named entity 'hairpin' named entity 'titers' named entity 'high' named entity 'RNAs' covid:arg/f9c39fa24056184c67ec47da4da93ba583c36e84 named entity 'cells' named entity 'effective' named entity 'expression' named entity 'high' named entity 'mortality' named entity 'real-time' named entity 'inhibitory' named entity 'effective' named entity 'detection' named entity 'viral' named entity 'cell proliferation' named entity 'TGE' named entity 'efficiency' named entity 'coronavirus' named entity 'swine' named entity 'plasmids' named entity 'RNA interference' named entity 'gene' named entity 'vomiting' named entity 'cell proliferation' named entity 'TGEV' named entity 'highly contagious' named entity 'diarrhea' named entity 'ORF' named entity 'virus' named entity 'ORF' named entity 'transmissible gastroenteritis coronavirus' named entity 'Lipofectamine' named entity 'genome' named entity 'vector' named entity 'animal studies' named entity 'ORF' named entity 'TGEV' named entity 'reverse genetics' named entity 'homology' named entity 'viral infection' named entity 'kDa' named entity 'fluorescence microscopy' named entity 'porcine' named entity 'gene' named entity 'gene' named entity 'TGEV' named entity 'shRNAs' named entity 'TGEV' named entity 'DMEM' named entity 'cell lines' named entity 'feline coronavirus' named entity 'virus' named entity 'real-time RT-PCR' named entity 'canine coronavirus' named entity 'transfected' named entity 'viral replication' named entity 'hydrophobic' named entity 'gene' named entity 'shRNAs' named entity 'TGEV' named entity 'negative control' named entity 'siRNAs' named entity 'GFP' named entity 'shRNAs' named entity 'genome' named entity 'RNAi' named entity 'neomycin' named entity 'viral genome' named entity 'RNAi'

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