About: Simultaneous Concentration of Bovine Viruses and Agricultural Zoonotic Bacteria from Water Using Sodocalcic Glass Wool Filters

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About: Simultaneous Concentration of Bovine Viruses and Agricultural Zoonotic Bacteria from Water Using Sodocalcic Glass Wool Filters Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Simultaneous Concentration of Bovine Viruses and Agricultural Zoonotic Bacteria from Water Using Sodocalcic Glass Wool Filters
Creator	Gerba, Charles Abd-Elmaksoud, Sherif Akrum, • Borchardt, Mark Jokela, William Spencer, Susan Tamimi, H
topic	covid:f92be2aecf981993b29cd04b3e25a6fdef1ed4d9#this
Source	Medline; PMC
abstract	Infiltration and runoff from manured agricultural fields can result in livestock pathogens reaching groundwater and surface waters. Here, we measured the effectiveness of glass wool filters to simultaneously concentrate enteric viruses and bacteria of bovine origin from water. The recovery efficiencies were determined for bovine viral diarrhea virus types 1 and 2, bovine rotavirus group A, bovine coronavirus, poliovirus Sabin III, toxigenic Escherichia coli ,and Campylobacter jejuni seeded into water with three different turbidity levels (0.5, 215, and 447 NTU). Twenty liters of dechlorinated tap water (pH 7) were seeded with the test organisms, and then passed through a glass wool filter using a peristaltic pump (flow rate = 1 liter min(−1)). Retained organisms were eluted from the filters by passing beef extract-glycine buffer (pH 9.5) in the direction opposite of sample flow. Recovered organisms were enumerated by qPCR except for C. jejuni, which was quantified by culture. Mean recovery efficiencies ranged from 55 to 33 % for the bacteria and 58 to 16 % for the viruses. Using bootstrapping techniques combined with Analysis of Variance, recovery efficiencies were found to differ among the pathogen types tested at the two lowest turbidity levels; however, for a given pathogen type turbidity did not affect recovery except for C. jejuni. Glass wool filtration is a cost-effective method for concentrating several waterborne pathogens of bovine origin simultaneously, although recovery may be low for some specific taxa such as bovine viral diarrhea virus 1.
has issue date	2014-07-25(xsd:dateTime)
bibo:doi	10.1007/s12560-014-9159-z
bibo:pmid	25059211
has license	no-cc
sha1sum (hex)	f92be2aecf981993b29cd04b3e25a6fdef1ed4d9
schema:url	https://doi.org/10.1007/s12560-014-9159-z
resource representing a document's title	Simultaneous Concentration of Bovine Viruses and Agricultural Zoonotic Bacteria from Water Using Sodocalcic Glass Wool Filters
has PubMed Central identifier	PMC7091103
has PubMed identifier	25059211
schema:publication	Food Environ Virol
resource representing a document's body	covid:f92be2aecf981993b29cd04b3e25a6fdef1ed4d9#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/f92be2aecf981993b29cd04b3e25a6fdef1ed4d9
is schema:about of	named entity 'group A' named entity 'affect' named entity 'recovery' named entity 'Escherichia coli' named entity 'bovine' named entity 'qPCR' named entity 'origin' named entity 'flow rate' named entity 'viruses' named entity 'efficiencies' named entity 'Glass' named entity 'VIRUSES' named entity 'MEASURED' named entity 'EFFECTIVENESS' named entity 'LOWEST' named entity 'AGRICULTURAL FIELDS' named entity 'PERISTALTIC PUMP ' named entity 'COST' named entity 'ENUMERATED' named entity 'TECHNIQUES' named entity 'III' named entity 'TURBIDITY' named entity '9.5' named entity 'LIVESTOCK' named entity '215' named entity 'CULTURE' covid:arg/f92be2aecf981993b29cd04b3e25a6fdef1ed4d9 named entity 'SIMULTANEOUS' named entity 'CONCENTRATION OF' named entity 'BACTERIA ' named entity 'AGRICULTURAL' named entity 'ENTERIC' named entity 'ROTAVIRUS GROUP A' named entity 'DID' named entity 'WATER' named entity 'FILTERS' named entity 'VIRUS TYPES' named entity 'FILTER' named entity 'LEVELS' named entity 'SAMPLE' named entity 'COMBINED' named entity 'USING' named entity 'VIRUSES' named entity 'GLASS WOOL' named entity 'ZOONOTIC BACTERIA' named entity 'WATERS' named entity 'MAY BE' named entity 'BOVINE' named entity 'DETERMINED' named entity 'METHOD' named entity 'SABIN' named entity 'GIVEN' named entity 'TAP WATER' named entity 'CONCENTRATE' named entity 'GLYCINE' named entity 'PATHOGEN' named entity 'BOVINE' named entity 'DIFFERENT' named entity 'BACTERIA ' named entity 'ESCHERICHIA COLI' named entity 'BOVINE ROTAVIRUS' named entity 'BOVINE CORONAVIRUS' named entity 'BEEF EXTRACT' named entity 'OPPOSITE' named entity 'INFILTRATION' named entity 'LITER' named entity 'FILTERS' named entity 'EXCEPT FOR' named entity 'TYPE' named entity 'AFFECT' named entity 'FOUND' named entity 'SPECIFIC' named entity 'RUNOFF'

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