About: Single-cell RNA-seq and V(D)J profiling of immune cells in COVID-19 patients

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About: Single-cell RNA-seq and V(D)J profiling of immune cells in COVID-19 patients Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Single-cell RNA-seq and V(D)J profiling of immune cells in COVID-19 patients
Creator	Chen, Wei Wu, Qian Zhou, Wei Ma, Qiang Chi, Xiangyang Dong, Yunzhu Fan, Hongyan Liu, Jianwei Yang, Yilong Ding, Wenyu Fan, Xiaoying Fang, Ting Ma, Wenji Wang, Xiaoqun Yin, Chonghai Zhang, Mengyao Zhong, Suijuan Zuo, Zhentao
Source	MedRxiv
abstract	Coronavirus disease 2019 (COVID-19) has caused over 220,000 deaths so far and is still an ongoing global health problem. However, the immunopathological changes of key types of immune cells during and after virus infection remain unclear. Here, we enriched CD3+ and CD19+ lymphocytes from peripheral blood mononuclear cells of COVID-19 patients (severe patients and recovered patients at early or late stages) and healthy people (SARS-CoV-2 negative) and revealed transcriptional profiles and changes in these lymphocytes by comprehensive single-cell transcriptome and V(D)J recombination analyses. We found that although the T lymphocytes were decreased in the blood of patients with virus infection, the remaining T cells still highly expressed inflammatory genes and persisted for a while after recovery in patients. We also observed the potential transition from effector CD8 T cells to central memory T cells in recovered patients at the late stage. Among B lymphocytes, we analyzed the expansion trajectory of a subtype of plasma cells in severe COVID-19 patients and traced the source as atypical memory B cells (AMBCs). Additional BCR and TCR analyses revealed a high level of clonal expansion in patients with severe COVID-19, especially of B lymphocytes, and the clonally expanded B cells highly expressed genes related to inflammatory responses and lymphocyte activation. V-J gene usage and clonal types of higher frequency in COVID-19 patients were also summarized. Taken together, our results provide crucial insights into the immune response against patients with severe COVID-19 and recovered patients and valuable information for the development of vaccines and therapeutic strategies.
has issue date	2020-05-27(xsd:dateTime)
bibo:doi	10.1101/2020.05.24.20101238
has license	medrxiv
sha1sum (hex)	f85cd535c65ed6ee4bc85647bfa0648815c0fab0
schema:url	https://doi.org/10.1101/2020.05.24.20101238
resource representing a document's title	Single-cell RNA-seq and V(D)J profiling of immune cells in COVID-19 patients
resource representing a document's body	covid:f85cd535c65ed6ee4bc85647bfa0648815c0fab0#body_text
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