About: miR‐193b represses influenza A virus infection by inhibiting Wnt/β‐catenin signalling

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About: miR‐193b represses influenza A virus infection by inhibiting Wnt/β‐catenin signalling Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	miR‐193b represses influenza A virus infection by inhibiting Wnt/β‐catenin signalling
Creator	Wang, Yang Yang, Xiaoyun Bamunuarachchi, Gayan Huang, Chaoqun Zhu, Zhengyu Liu, Liu, \| Xu, Lan Lin, \| Zhao, Chunling Liang, Yurong Xu, Dao Correspondence, Lin Lakmini, \| Senavirathna, Kumari
source	Medline; PMC
abstract	Due to an increasing emergence of new and drug‐resistant strains of the influenza A virus (IAV), developing novel measures to combat influenza is necessary. We have previously shown that inhibiting Wnt/β‐catenin pathway reduces IAV infection. In this study, we aimed to identify antiviral human microRNAs (miRNAs) that target the Wnt/β‐catenin signalling pathway. Using a miRNA expression library, we identified 85 miRNAs that up‐regulated and 20 miRNAs that down‐regulated the Wnt/β‐catenin signalling pathway. Fifteen miRNAs were validated to up‐regulate and five miRNAs to down‐regulate the pathway. Overexpression of four selected miRNAs (miR‐193b, miR‐548f‐1, miR‐1‐1, and miR‐509‐1) that down‐regulated the Wnt/β‐catenin signalling pathway reduced viral mRNA, protein levels in A/PR/8/34‐infected HEK293 cells, and progeny virus production. Overexpression of miR‐193b in lung epithelial A549 cells also resulted in decreases of A/PR/8/34 infection. Furthermore, miR‐193b inhibited the replication of various strains, including H1N1 (A/PR/8/34, A/WSN/33, A/Oklahoma/3052/09) and H3N2 (A/Oklahoma/309/2006), as determined by a viral reporter luciferase assay. Further studies revealed that β‐catenin was a target of miR‐193b, and β‐catenin rescued miR‐193b‐mediated suppression of IAV infection. miR‐193b induced G0/G1 cell cycle arrest and delayed vRNP nuclear import. Finally, adenovirus‐mediated gene transfer of miR‐193b to the lung reduced viral load in mice challenged by a sublethal dose of A/PR/8/34. Collectively, our findings suggest that miR‐193b represses IAV infection by inhibiting Wnt/β‐catenin signalling.
has issue date	2019-01-25(xsd:dateTime)
bibo:doi	10.1111/cmi.13001
bibo:pmid	30650225
has license	no-cc
sha1sum (hex)	f7ee7afaf51dae79d4b480b8b9aa926b03f638c0
schema:url	https://doi.org/10.1111/cmi.13001
resource representing a document's title	miR‐193b represses influenza A virus infection by inhibiting Wnt/β‐catenin signalling
has PubMed Central identifier	PMC6459727
has PubMed identifier	30650225
schema:publication	Cell Microbiol
resource representing a document's body	covid:f7ee7afaf51dae79d4b480b8b9aa926b03f638c0#body_text
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