About: A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification

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About: A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification
Creator	Li, Hui Wang, Chen Dillon, Natalie Wong, Freda Pui-Fan Chan, Paul Cheung, Albert Fung, Yin-Wan Lau, Collins, Richard Lin, Sau-Wah Lok, Ting Tam, Siu-Lun Yu, -H Yu, -Hoi
Source	Elsevier; Medline; PMC
abstract	Abstract An enhanced polymerase chain reaction (PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-CoV) was developed in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. Clinical samples were collected from 120 patients diagnosed as suspected or probable SARS cases and analyzed by conventional PCR followed by agarose gel electrophoresis, conventional TaqMan real-time PCR, and our enhanced TaqMan real-time PCR assays. An amplicon of the size expected from SARS-CoV was obtained from 28/120 samples using the enhanced real-time PCR method. Conventional PCR and real-time PCR alone identified fewer SARS-CoV positive cases. Results were confirmed by viral culture in 3/28 cases. The limit of detection of the enhanced real-time PCR method was 102-fold higher than the standard real-time PCR assay and 107-fold higher than conventional PCR methods. The increased sensitivity of the assay may help control the spread of the disease during future SARS outbreaks.
has issue date	2003-12-26(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2003.11.064
bibo:pmid	14652014
has license	els-covid
sha1sum (hex)	f74923b3ce82c984a7ae3e0c2754c9e33c60554f
schema:url	https://doi.org/10.1016/j.bbrc.2003.11.064
resource representing a document's title	A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification
has PubMed Central identifier	PMC7111096
has PubMed identifier	14652014
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:f74923b3ce82c984a7ae3e0c2754c9e33c60554f#body_text
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