About: The virus-specific intracellular RNA species of two murine coronaviruses: MHV-A59 and MHV-JHM

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About: The virus-specific intracellular RNA species of two murine coronaviruses: MHV-A59 and MHV-JHM Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The virus-specific intracellular RNA species of two murine coronaviruses: MHV-A59 and MHV-JHM
Creator	Leibowitz, Julian Bond4, Clifford Wilhelmsen, Kirk
Source	Elsevier; Medline; PMC
abstract	Abstract Seven virus-specific, polyadenylated RNA species have been identified in mouse cells infected with the murine coronaviruses MHV-A59 (A59V) or MHV-JHM (JHMV). MHV-infected 17CL·1 cells were labeled with [32P]orthophosphate in the presence of actinomycin D and the cytoplasmic RNA was extracted and analyzed by agarose gel electrophoresis. These RNA species range in size from 6.3 × 105 to 6.1 × 106 daltons. The A59V and JHMV-specific RNAs have identical molecular weights and comigrate in agarose gels. The largest intracellular RNA species is identical to RNA isolated from purified virions, as determined by agarose gel electrophoresis and oligonucleotide fingerprint studies of ribonuclease T1 digests. Oligonucleotide fingerprints of the six subgenomic RNAS show that the sequences they contain are present in virion RNA, confirming their virus-specific nature. The fingerprinting studies also demonstrate that the six subgenomic RNA species make up a nested set. The sequences present in each RNA species are also present in all larger RNA species. These larger RNAs also contain additional sequences consistent with their greater size. The subgenomic RNAs fulfull many of the criteria for mRNAs. Possible mechanisms for generating these RNAs are discussed.
has issue date	1981-10-15(xsd:dateTime)
bibo:doi	10.1016/0042-6822(81)90250-6
bibo:pmid	7281517
has license	els-covid
sha1sum (hex)	f66156b6a380cb402e6006102ab714fab10e9170
schema:url	https://doi.org/10.1016/0042-6822%2881%2990250-6
resource representing a document's title	The virus-specific intracellular RNA species of two murine coronaviruses: MHV-A59 and MHV-JHM
has PubMed Central identifier	PMC7131044
has PubMed identifier	7281517
schema:publication	Virology
resource representing a document's body	covid:f66156b6a380cb402e6006102ab714fab10e9170#body_text
is schema:about of	named entity 'studies' named entity 'intracellular' named entity 'RNA' named entity 'MHV' named entity 'ADDITIONAL' named entity 'DETERMINED BY' named entity 'PRESENCE OF' named entity 'PURIFIED' named entity 'L CELLS' named entity '17CL' named entity 'INFECTED' covid:arg/f66156b6a380cb402e6006102ab714fab10e9170 named entity 'INTRACELLULAR' named entity 'GREATER' named entity 'IDENTICAL' named entity 'CELLS' named entity 'HAVE' named entity 'NATURE' named entity 'STUDIES' named entity 'LARGEST' named entity 'FINGERPRINT' named entity 'AGAROSE GEL ELECTROPHORESIS' named entity 'ISOLATED' named entity 'FINGERPRINTING' named entity 'THEIR' named entity 'CORONAVIRUSES' named entity 'LABELED' named entity '6.3' named entity 'MRNAS' named entity 'SPECIFIC' named entity 'SPECIES' named entity 'VIRION' named entity 'POLYADENYLATED RNA' named entity 'VIRIONS' named entity 'OLIGONUCLEOTIDE' named entity 'RNA' named entity 'SPECIES' named entity 'SEVEN' named entity 'ANALYZED' named entity 'FINGERPRINTS' named entity 'VIRUS' named entity 'IDENTIFIED' named entity 'CRITERIA' named entity 'MHV' named entity 'A59' named entity 'LARGER' named entity 'AGAROSE' named entity 'THESE' named entity 'MURINE' named entity 'CORONAVIRUSES' named entity 'SPECIFIC' named entity 'VIRUS' named entity 'RANGE' named entity 'PRESENT' named entity 'ACTINOMYCIN D' named entity 'POSSIBLE' named entity 'MAKE' named entity 'MURINE' named entity 'GELS' named entity 'CONSISTENT WITH' named entity 'MECHANISMS' named entity 'MOLECULAR WEIGHTS' named entity 'NESTED' named entity 'MOUSE' named entity 'SIZE' named entity 'EXTRACTED' named entity 'CONTAIN' named entity 'SET' named entity 'SEQUENCES' named entity 'CONFIRMING' named entity 'INTRACELLULAR' named entity 'A59' named entity 'GENERATING' named entity 'RIBONUCLEASE T1' named entity 'fingerprinting' named entity 'range' named entity 'nature' named entity 'labeled' named entity 'demonstrate'

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