About: RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations

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About: RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations
Creator	Wattrang, Eva Chen, Jun Buitenhuis, Bart Gualdi, Valentina Hamzić, Edin Mach, Núria Minozzi, Guilietta Strozzi, Francesco Williams, John Dalgaard, Tina Kjaerup, Rikke Risdahl, Helle
Source	Medline; PMC
abstract	BACKGROUND: Avian infectious bronchitis is a highly contagious disease of the upper-respiratory tract caused by infectious bronchitis virus (IBV). Understanding the molecular mechanisms involved in the interaction between innate and adaptive immune responses to IBV infection is a crucial element for further improvements in strategies to control IB. To this end, two chicken lines, selected for high (L10H line) and low (L10L line) serum concentration of mannose-binding lectin (MBL) were studied. In total, 32 birds from each line were used. Sixteen birds from each line were infected with IBV and sixteen were left uninfected. Eight uninfected and infected birds from each line were euthanized at 1 and 3 weeks post infection. RNA sequencing was performed on spleen samples from all 64 birds and differential gene expression analysis was performed for four comparisons: L10L line versus L10H line for uninfected birds at weeks 1 and 3, respectively, and in the same way for infected birds. Functional analysis was performed using Gene Ontology (GO) Immune System Process terms specific for Gallus gallus. RESULTS: Comparing uninfected L10H and L10L birds, we identified 1698 and 1424 differentially expressed (DE) genes at weeks 1 and 3, respectively. For the IBV-infected birds, 1934 and 866 DE genes were identified between the two lines at weeks 1 and 3, respectively. The two most enriched GO terms emerging from the comparison of uninfected birds between the two lines were “Lymphocyte activation involved in immune response” and “Somatic recombination of immunoglobulin genes involved in immune response” at weeks 1 and 3, respectively. When comparing IBV-infected birds between the two lines, the most enriched GO terms were “Alpha-beta T cell activation” and “Positive regulation of leukocyte activation” at weeks 1 and 3, respectively. CONCLUSIONS: Healthy birds from the two lines showed significant differences in expression profiles for subsets of adaptive and innate immunity-related genes, whereas comparison of the IBV-infected birds from the two lines showed differences in expression of immunity-related genes involved in T cell activation and proliferation. The observed transcriptome differences between the two lines indicate that selection for MBL had influenced innate as well as adaptive immunity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2403-1) contains supplementary material, which is available to authorized users.
has issue date	2016-01-27(xsd:dateTime)
bibo:doi	10.1186/s12864-016-2403-1
bibo:pmid	26819139
has license	cc-by
sha1sum (hex)	f5f1cd43740b5b6eca8b3cf2714fc0854a746519
schema:url	https://doi.org/10.1186/s12864-016-2403-1
resource representing a document's title	RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations
has PubMed Central identifier	PMC4729133
has PubMed identifier	26819139
schema:publication	BMC Genomics
resource representing a document's body	covid:f5f1cd43740b5b6eca8b3cf2714fc0854a746519#body_text
is schema:about of	named entity 'DIFFERENT' named entity 'BASED' named entity 'CHICKENS' named entity 'GO terms' named entity 'concentration' named entity 'expression' named entity 'lines' named entity 'Eight' named entity 'weeks' named entity 'Background' named entity 'immune response' named entity 'When' named entity 'TRANSCRIPTOME' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'FOLLOWING' named entity '3 WEEKS' named entity 'TO CONTROL' named entity 'CHICKEN' named entity 'WAY' named entity 'SPECIFIC' named entity 'T CELL ACTIVATION' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'IMMUNE SYSTEM PROCESS' named entity 'CAUSED BY' named entity 'SERUM CONCENTRATION' named entity 'EUTHANIZED' named entity 'ENRICHED' named entity 'FUNCTIONAL ANALYSIS' named entity 'UNINFECTED' named entity 'LOW' named entity 'WEEKS' named entity 'SELECTED' named entity 'ANALYSIS' named entity 'IDENTIFIED' named entity 'MANNOSE-BINDING LECTIN' named entity 'BIRDS' named entity '866' named entity 'GENE ONTOLOGY' named entity 'IS A' named entity 'IMMUNOGLOBULIN GENES' named entity 'PERFORMED' named entity 'INTERACTION' named entity 'TOTAL' named entity 'GALLUS GALLUS' named entity 'INFECTED' named entity 'RESULTS' named entity 'LINE' named entity 'DIFFERENTIAL GENE EXPRESSION ANALYSIS' named entity 'TERMS' named entity 'HIGH' named entity 'RECOMBINATION' named entity 'STRATEGIES' named entity 'REGULATION OF LEUKOCYTE ACTIVATION' named entity 'ELEMENT' named entity 'COMPARING' named entity 'END' covid:arg/f5f1cd43740b5b6eca8b3cf2714fc0854a746519 named entity 'ACTIVATION' named entity 'MECHANISMS' named entity 'SPLEEN' named entity 'RNA SEQUENCING' named entity 'SELECTED' named entity 'SERUM' named entity 'ADAPTIVE IMMUNE RESPONSES' named entity 'MOLECULAR' named entity 'MANNOSE-BINDING LECTIN' named entity 'USED' named entity 'IMMUNE RESPONSE' named entity 'COMPARISON' named entity 'SAMPLES'

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