About: Enteral delivery of proteins enhances the expression of proteins involved in the cytoskeleton and protein biosynthesis in human duodenal mucosa

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Enteral delivery of proteins enhances the expression of proteins involved in the cytoskeleton and protein biosynthesis in human duodenal mucosa Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Enteral delivery of proteins enhances the expression of proteins involved in the cytoskeleton and protein biosynthesis in human duodenal mucosa
Creator	Déchelotte, Pierre Bertrand, Julien Cailleux, Anne-Françoise Chan, Philippe Coquard, Aude Goichon, Alexis Coe¨ffier, Moı¨se Lecleire, Stéphane Vaudry, David
Source	PMC
abstract	Background: Amino acids are well known to be key effectors of gut protein turnover. We recently reported that enteral delivery of proteins markedly stimulated global duodenal protein synthesis in carbohydrate-fed healthy humans, but specifically affected proteins remain unknown. Objective: We aimed to assess the influence of an enteral protein supply on the duodenal mucosal proteome in carbohydrate-fed humans. Design: Six healthy volunteers received for 5 h, on 2 occasions and in random order, either an enteral infusion of maltodextrins alone (0.25 g · kg(−1) · h(−1)) mimicking the fed state or maltodextrins with a protein powder (0.14 g proteins · kg(−1) · h(−1)). Endoscopic duodenal biopsy specimens were then collected and frozen until analysis. A 2-dimensional polyacrylamide gel electrophoresis–based comparative proteomics analysis was then performed, and differentially expressed proteins (at least ±1.5-fold change; Student’s t test, P < 0.05) were identified by mass spectrometry. Protein expression changes were confirmed by Western blot analysis. Results: Thirty-two protein spots were differentially expressed after protein delivery compared with maltodextrins alone: 28 and 4 spots were up- or downregulated, respectively. Among the 22 identified proteins, 11 upregulated proteins were involved either in the cytoskeleton (ezrin, moesin, plastin 1, lamin B1, vimentin, and β-actin) or in protein biosynthesis (glutamyl-prolyl–transfer RNA synthetase, glutaminyl–transfer RNA synthetase, elongation factor 2, elongation factor 1δ, and eukaryotic translation and initiation factor 3 subunit f). Conclusions: Enteral delivery of proteins altered the duodenal mucosal proteome and mainly stimulated the expression of proteins involved in cytoskeleton and protein biosynthesis. These results suggest that protein supply may affect intestinal morphology by stimulating actin cytoskeleton remodeling. This trial was registered at clinicaltrials.gov as NCT01254110.
has issue date	2015-06-24(xsd:dateTime)
bibo:doi	10.3945/ajcn.114.104216
bibo:pmid	26109581
has license	no-cc
sha1sum (hex)	f54a1b62f5070c0aa410497cec838a42a950c8a5
schema:url	https://doi.org/10.3945/ajcn.114.104216
resource representing a document's title	Enteral delivery of proteins enhances the expression of proteins involved in the cytoskeleton and protein biosynthesis in human duodenal mucosa
has PubMed Central identifier	PMC7109707
has PubMed identifier	26109581
schema:publication	Am J Clin Nutr
resource representing a document's body	covid:f54a1b62f5070c0aa410497cec838a42a950c8a5#body_text
is schema:about of	named entity 'downregulated' named entity 'Results' named entity 'effectors' named entity 'spots' named entity 'protein biosynthesis' named entity 'cytoskeleton' named entity 'PROTEINS' named entity 'PERFORMED' named entity 'STIMULATED' named entity 'IDENTIFIED' named entity 'CONFIRMED BY' named entity 'PROTEIN EXPRESSION' named entity 'SYNTHETASE' named entity 'EITHER' named entity 'ORDER' named entity 'RANDOM' named entity 'PROTEOME' named entity '0.05' named entity 'PROTEINS' named entity 'PROTEIN DELIVERY' named entity 'FED' named entity 'HEALTHY' named entity 'THIRTY' named entity 'SPECIMENS' named entity 'THESE' named entity 'BUT' covid:arg/f54a1b62f5070c0aa410497cec838a42a950c8a5 named entity 'DUODENAL MUCOSA' named entity 'PROTEIN ' named entity 'EXPRESSION' named entity 'T TEST' named entity 'HUMANS' named entity 'MOESIN' named entity 'VIMENTIN' named entity 'INTESTINAL MORPHOLOGY' named entity 'GUT' named entity 'REGISTERED' named entity 'ENTERAL' named entity 'CYTOSKELETON' named entity 'INVOLVED' named entity 'PROTEIN BIOSYNTHESIS' named entity 'DELIVERY' named entity 'ACTIN CYTOSKELETON REMODELING' named entity 'MUCOSAL' named entity 'WESTERN BLOT ANALYSIS' named entity 'CLINICALTRIALS.GOV' named entity 'MARKEDLY' named entity 'AFFECTED' named entity 'CARBOHYDRATE' named entity 'DUODENAL BIOPSY' named entity 'SPOTS' named entity 'SUBUNIT' named entity 'HUMAN' named entity 'ENTERAL' named entity 'MASS SPECTROMETRY' named entity 'PROTEIN TURNOVER' named entity 'SUGGEST' named entity 'INFUSION' named entity 'G PROTEINS' named entity 'PROTEIN BIOSYNTHESIS' named entity 'RECEIVED' named entity 'FED STATE' named entity 'INITIATION FACTOR' named entity 'PLASTIN 1' named entity 'EXPRESSION' named entity 'ANALYSIS' named entity 'DELIVERY' named entity 'FROZEN' named entity 'ASSESS' named entity 'INVOLVED' named entity 'AMINO ACIDS' named entity 'ALTERED' named entity 'CONCLUSIONS'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software