About: Prediction and biochemical analysis of putative cleavage sites of the 3C-like protease of Middle East respiratory syndrome coronavirus

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About: Prediction and biochemical analysis of putative cleavage sites of the 3C-like protease of Middle East respiratory syndrome coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Prediction and biochemical analysis of putative cleavage sites of the 3C-like protease of Middle East respiratory syndrome coronavirus
Creator	Wang, Yi Chen, Yu Wang, Min Guo, Deyin Su, Ceyang Wu, Andong Xu, Shan Zeng, Cong Huang, Xingyu
Source	Elsevier; Medline; PMC
abstract	Abstract Coronavirus 3C-like protease (3CLpro) is responsible for the cleavage of coronaviral polyprotein 1a/1ab (pp1a/1ab) to produce the mature non-structural proteins (nsps) of nsp4–16. The nsp5 of the newly emerging Middle East respiratory syndrome coronavirus (MERS-CoV) was identified as 3CLpro and its canonical cleavage sites (between nsps) were predicted based on sequence alignment, but the cleavability of these cleavage sites remains to be experimentally confirmed and putative non-canonical cleavage sites (inside one nsp) within the pp1a/1ab awaits further analysis. Here, we proposed a method for predicting coronaviral 3CLpro cleavage sites which balances the prediction accuracy and false positive outcomes. By applying this method to MERS-CoV, the 11 canonical cleavage sites were readily identified and verified by the biochemical assays. The Michaelis constant of the canonical cleavage sites of MERS-CoV showed that the substrate specificity of MERS-CoV 3CLpro is relatively conserved. Interestingly, nine putative non-canonical cleavage sites were predicted and three of them could be cleaved by MERS-CoV nsp5. These results pave the way for identification and functional characterization of new nsp products of coronaviruses.
has issue date	2015-10-02(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2015.05.018
bibo:pmid	26036787
has license	els-covid
sha1sum (hex)	f500549a408ef72f48bc8b64cd5d34e43b3b2241
schema:url	https://doi.org/10.1016/j.virusres.2015.05.018
resource representing a document's title	Prediction and biochemical analysis of putative cleavage sites of the 3C-like protease of Middle East respiratory syndrome coronavirus
has PubMed Central identifier	PMC7114542
has PubMed identifier	26036787
schema:publication	Virus Research
resource representing a document's body	covid:f500549a408ef72f48bc8b64cd5d34e43b3b2241#body_text
is schema:about of	named entity 'PREDICTION' named entity 'predicted' named entity 'The' named entity 'canonical' named entity 'CLEAVAGE' named entity 'LIKE' named entity 'BUT' named entity 'NSP5' named entity 'MATURE' named entity 'METHOD' named entity 'PROTEASE' named entity 'RESULTS' named entity 'WAY' named entity 'OUTCOMES' named entity 'COULD BE' named entity 'CORONAVIRUSES' named entity 'PAVE' named entity 'IDENTIFIED' named entity 'MICHAELIS CONSTANT' named entity 'BASED' named entity 'CONSERVED' named entity 'SEQUENCE ALIGNMENT' named entity 'SITES' named entity 'CHARACTERIZATION' named entity 'CLEAVAGE' named entity 'FUNCTIONAL' named entity 'PREDICTION' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'BIOCHEMICAL ANALYSIS' named entity 'NEW' named entity 'SITES' named entity 'PROTEASE' named entity 'BALANCES' named entity 'CLEAVED' named entity 'TO PRODUCE' named entity 'PRODUCTS' named entity 'THESE' named entity 'PROPOSED' named entity 'CONFIRMED' named entity 'NSPS' named entity 'VERIFIED' named entity 'NON-STRUCTURAL PROTEINS' named entity 'PREDICTING' named entity 'ANALYSIS' named entity 'BIOCHEMICAL' named entity 'SUBSTRATE SPECIFICITY' named entity 'NSP' named entity 'NON-' named entity 'APPLYING' named entity 'CORONAVIRUS' named entity 'NEWLY' named entity 'PP1A' named entity 'NSP4' named entity 'LIKE' named entity 'ITS' named entity 'FALSE POSITIVE' named entity 'ACCURACY' named entity 'RESPONSIBLE' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'HERE' named entity 'PREDICTED' named entity 'IDENTIFICATION' named entity 'POLYPROTEIN' covid:arg/f500549a408ef72f48bc8b64cd5d34e43b3b2241 named entity 'The' named entity 'cleavage' named entity 'cleavage' named entity 'sites' named entity 'MERS-CoV' named entity 'non-canonical' named entity 'based' named entity 'biochemical analysis' named entity 'nsp'

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