About: The Influence of Age on Steroidogenic Enzyme Activities of the Rat Adrenal Gland: Enhanced Expression of Cholesterol Side-Chain Cleavage Activity(*)

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About: The Influence of Age on Steroidogenic Enzyme Activities of the Rat Adrenal Gland: Enhanced Expression of Cholesterol Side-Chain Cleavage Activity(*) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Influence of Age on Steroidogenic Enzyme Activities of the Rat Adrenal Gland: Enhanced Expression of Cholesterol Side-Chain Cleavage Activity(*)
Creator	Azhar, Salman Butte, John Popplewellf, Philip
Source	PMC
abstract	The ability of isolated adrenocortical cells to secrete corticosterone in response to ACTH challenge declines as rats age, but the site or mechanism(s) of this impairment is still unknown. To test the functionality of steroidogenic capacity per se, we measured the key enzyme activities involved in corticosterone biosynthesis. We also measured the mitochondrial cytochrome P-450 content and nonsteroidogenic enzymes specific for subcellular fractions. Mitochondria and microsomal fractions were isolated from the adrenals of 2-, 12-, and 18- month-old animals and used for various enzyme measurements. Mitochondrial side-chain cleavage enzyme activity (nanomoles per min mg protein(-1)) increased from a mean of 0.43 ± 0.06 in 2-month-old rats to 1.26 ± 0.11 and 1.51 ± 0.06 in 12- and 18-month old rats, respectively. After incubation with 5- cholesten-3j8,25-diol (25-hydroxycholesterol; 25 μg/ml) sidechain cleave activity rose to 5.0 ± 0.6, 12.4 ± 1.2, and 16 ± 1.4 nmol min(-1) mg protein(-1) in adrenal mitochondrial fractions from 2-, 12-, and 18-month-old rats, respectively. In contrast, mitochondrial cytochrome P-450 content did not vary with advancing age. Microsomal δ(5)-3β-hyroxysteroid dehydrogenase-δ(5)-δ(4)- isomerase activities were similar in 2- and 12-month-old rats, but 21-hydroxylase (nanomoles per min mg protein(-1)) activity was significantly increased in 12-month-old rats (2-month-old, 5.2 ± 0.2; 12-month-old, 7.7 ± 0.5). Finally, mitochondrial 11β- hydroxylase was comparable in both age groups. In addition, activities of mitochondrial nonsteroidogenic enzymes, such as monoamine oxidase, amytal insensitive NADH cytochrome c reductase, cytochrome c oxidase, succinate dehydrogenase, and malate dehydrogenase, did not change with age. It appears from the evidence presented that the activities of the steroidogenic enzymes are not responsible for the diminished capacity in corticosterone production seen with aging in the rat. (Endocrinology120: 2521–2528,1987)
has issue date	1987-06-01(xsd:dateTime)
bibo:doi	10.1210/endo-120-6-2521
bibo:pmid	3569141
has license	no-cc
sha1sum (hex)	f4cb5c1bc8d5199a3bbee98951395f015eccdef9
schema:url	https://doi.org/10.1210/endo-120-6-2521
resource representing a document's title	The Influence of Age on Steroidogenic Enzyme Activities of the Rat Adrenal Gland: Enhanced Expression of Cholesterol Side-Chain Cleavage Activity(*)
has PubMed Central identifier	PMC7108490
has PubMed identifier	3569141
schema:publication	Endocrinology
resource representing a document's body	covid:f4cb5c1bc8d5199a3bbee98951395f015eccdef9#body_text
is schema:about of	named entity 'isolated' named entity 'Mitochondria' named entity 'microsomal' named entity 'measured' named entity 'steroidogenic' named entity 'activities' named entity 'secrete' named entity 'Adrenal' named entity 'Mitochondria' named entity 'enzyme' named entity 'microsomal' named entity 'steroidogenic' named entity 'cytochrome P-450' named entity 'Cleavage' named entity 'Cholesterol' named entity 'side-chain' named entity 'microsomal' named entity 'dichloromethane' named entity 'microfilaments' named entity 'cholesterol ester' named entity 'adrenal gland' named entity 'protein' named entity 'cleavage' named entity 'carrier proteins' named entity 'potassium phosphate' named entity 'virus' named entity 'ACTH' named entity 'glucose-6-phosphate dehydrogenase' named entity 'corticosterone' named entity 'corticosterone' named entity 'adrenal' named entity 'steroidogenic' named entity 'enzyme' named entity 'senescent' named entity 'senescent' named entity 'side-chain' named entity 'side-chain' named entity 'deoxycorticosterone' named entity 'sterol' named entity 'oxidized' named entity 'mitochondria' named entity 'side-chain cleavage enzyme' named entity 'progesterone' named entity 'sodium' named entity 'side-chain' named entity 'cleavage' named entity 'progesterone' named entity 'pregnenolone' named entity 'Fatty acid' named entity 'corticosterone' named entity 'protein' named entity 'cholesterol' named entity 'adrenals' named entity 'mice' named entity 'adrenals' named entity 'cholesterol' named entity 'enzyme activity' named entity 'fatty acid' named entity 'testes' named entity 'senescent' named entity 'cleavage' named entity 'corticosterone' named entity 'adrenocortical cells' named entity 'enzyme' named entity 'NADH' named entity 'Steroid' named entity '5:1' named entity 'enzyme' named entity 'protein' named entity 'ether' named entity 'cytochrome' named entity 'adrenal' named entity 'glucose-6-phosphate' named entity 'mitochondria' named entity 'adrenal' named entity 'steroids' named entity 'NADH' named entity 'side-chain cleavage enzyme' named entity 'Denville'

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