About: Microarray analysis of MicroRNA expression in peripheral blood mononuclear cells of critically ill patients with influenza A (H1N1)

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About: Microarray analysis of MicroRNA expression in peripheral blood mononuclear cells of critically ill patients with influenza A (H1N1) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Microarray analysis of MicroRNA expression in peripheral blood mononuclear cells of critically ill patients with influenza A (H1N1)
Creator	Wang, Qi Cheng, Jun Song, Hao Guo, Yang Dai, Li Song, Rui Gao, Xuesong Li, Baoshun Liu, Shunai Zhang, Deli
Source	PMC
abstract	BACKGROUND: With concerns about the disastrous health and economic consequences caused by the influenza pandemic, comprehensively understanding the global host response to influenza virus infection is urgent. The role of microRNA (miRNA) has recently been highlighted in pathogen-host interactions. However, the precise role of miRNAs in the pathogenesis of influenza virus infection in humans, especially in critically ill patients is still unclear. METHODS: We identified cellular miRNAs involved in the host response to influenza virus infection by performing comprehensive miRNA profiling in peripheral blood mononuclear cells (PBMCs) from critically ill patients with swine-origin influenza pandemic H1N1 (2009) virus infection via miRNA microarray and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) assays. Receiver operator characteristic (ROC) curve analysis was conducted and area under the ROC curve (AUC) was calculated to evaluate the diagnostic accuracy of severe H1N1 influenza virus infection. Furthermore, an integrative network of miRNA-mediated host-influenza virus protein interactions was constructed by integrating the predicted and validated miRNA-gene interaction data with influenza virus and host-protein-protein interaction information using Cytoscape software. Moreover, several hub genes in the network were selected and validated by qRT-PCR. RESULTS: Forty-one significantly differentially expressed miRNAs were found by miRNA microarray; nine were selected and validated by qRT-PCR. QRT-PCR assay and ROC curve analyses revealed that miR-31, miR-29a and miR-148a all had significant potential diagnostic value for critically ill patients infected with H1N1 influenza virus, which yielded AUC of 0.9510, 0.8951 and 0.8811, respectively. We subsequently constructed an integrative network of miRNA-mediated host-influenza virus protein interactions, wherein we found that miRNAs are involved in regulating important pathways, such as mitogen-activated protein kinase signaling pathway, epidermal growth factor receptor signaling pathway, and Toll-like receptor signaling pathway, during influenza virus infection. Some of differentially expressed miRNAs via in silico analysis targeted mRNAs of several key genes in these pathways. The mRNA expression level of tumor protein T53 and transforming growth factor beta receptor 1 were found significantly reduced in critically ill patients, whereas the expression of Janus kinase 2, caspase 3 apoptosis-related cysteine peptidase, interleukin 10, and myxovirus resistance 1 were extremely increased in critically ill patients. CONCLUSIONS: Our data suggest that the dysregulation of miRNAs in the PBMCs of H1N1 critically ill patients can regulate a number of key genes in the major signaling pathways associated with influenza virus infection. These differentially expressed miRNAs could be potential therapeutic targets or biomarkers for severe influenza virus infection.
has issue date	2013-06-03(xsd:dateTime)
bibo:doi	10.1186/1471-2334-13-257
bibo:pmid	23731466
has license	cc-by
sha1sum (hex)	f3f1997f1d143697faaf7c1fc78684ae8a8b1d5d
schema:url	https://doi.org/10.1186/1471-2334-13-257
resource representing a document's title	Microarray analysis of MicroRNA expression in peripheral blood mononuclear cells of critically ill patients with influenza A (H1N1)
has PubMed Central identifier	PMC3679792
has PubMed identifier	23731466
schema:publication	BMC Infect Dis
resource representing a document's body	covid:f3f1997f1d143697faaf7c1fc78684ae8a8b1d5d#body_text
is schema:about of	named entity 'virus' named entity 'expression' named entity 'CONCERNS' named entity 'INFLUENZA VIRUS' named entity 'PATIENTS' named entity 'INTERACTIONS' named entity 'With' named entity 'miRNA' named entity 'pathogen-host interactions' named entity 'peripheral blood mononuclear cells' named entity 'critically ill' named entity 'Microarray analysis' named entity 'host response' named entity 'microRNA' named entity 'infection' named entity 'pathogen-host interactions' named entity 'influenza virus' named entity 'critically ill patients' named entity 'peripheral blood mononuclear cells' named entity 'influenza' named entity 'signaling pathways' named entity 'false discovery rate' named entity 'inflammation' named entity 'ROC analysis' named entity 'chemokine' named entity 'IL-13' named entity 'infection' named entity 'KEGG' named entity 'miR-155' named entity 'viral replication' named entity 'Agilent Technologies' named entity 'IL-12' named entity 'inflammatory response' named entity 'downregulation' named entity 'Biochip' named entity 'protein kinase' named entity 'influenza-like' named entity 'MAPK' named entity 'infection' named entity 'miRNA' named entity 'cytokines' named entity 'agonists' named entity 'type 2 diabetes' named entity 'expression profiles' named entity '5.1' named entity 'cell receptor' named entity 'septic shock' named entity 'viral pathogenesis' named entity 'CASP3' named entity 'miRNAs' named entity 'co-occurrence' named entity 'intensive care unit' named entity 'antiviral' named entity 'expression analysis' named entity 'mice' named entity 'MX1' named entity 'miRNAs' named entity 'influenza virus' named entity 'downregulated' named entity 'in-degree' named entity 'qRT-PCR' named entity 'dehydration' named entity 'Wnt signaling pathway' named entity 'pathogenesis' named entity 'Mx1' named entity 'influenza virus' named entity 'miR' named entity 'intensive care' named entity 'cell differentiation' named entity 'infection' named entity 'qRT-PCR' named entity 'RANTES'

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