About: Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads

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About: Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads
Creator	Wang, Quanyi Yang, Peng Pan, Yang Cui, Shujuan Zhang, Daitao Yan, Tingting Li, Heping Long, Luyao Ren #, Simei
Source	Medline; PMC
abstract	BACKGROUND: Corona Virus Disease-2019 (COVID-19) has spread widely throughout the world since the end of 2019. Nucleic acid testing (NAT) has played an important role in patient diagnosis and management of COVID-19. In some circumstances, thermal inactivation at 56 °C has been recommended to inactivate Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) before NAT. However, this procedure could theoretically disrupt nucleic acid integrity of this single-stranded RNA virus and cause false negatives in real-time polymerase chain reaction (RT-PCR) tests. METHODS: We investigated whether thermal inactivation could affect the results of viral NAT. We examined the effects of thermal inactivation on the quantitative RT-PCR results of SARS-CoV-2 particularly with regard to the rates of false-negative results for specimens carrying low viral loads. We additionally investigated the effects of different specimen types, sample preservation times and a chemical inactivation approach on NAT. RESULTS: Our work showed increased Ct values in specimens from diagnosed COVID-19 patients in RT-PCR tests after thermal incubation. Moreover, about half of the weak-positive samples (7 of 15 samples, 46.7%) were RT-PCR negative after heat inactivation in at least one parallel testing. The use of guanidinium-based lysis for preservation of these specimens had a smaller impact on RT-PCR results with fewer false negatives (2 of 15 samples, 13.3%) and significantly less increase in Ct values than heat inactivation. CONCLUSION: Thermal inactivation adversely affected the efficiency of RT-PCR for SARS-CoV-2 detection. Given the limited applicability associated with chemical inactivators, other approaches to ensure the overall protection of laboratory personnel need consideration.
has issue date	2020-04-04(xsd:dateTime)
bibo:doi	10.1093/clinchem/hvaa091
bibo:pmid	32246822
has license	no-cc
sha1sum (hex)	f37db64e5c752c16ecb31debb4cbc5db69ba1969
schema:url	https://doi.org/10.1093/clinchem/hvaa091
resource representing a document's title	Potential false-negative nucleic acid testing results for Severe Acute Respiratory Syndrome Coronavirus 2 from thermal inactivation of samples with low viral loads
has PubMed Central identifier	PMC7184485
has PubMed identifier	32246822
schema:publication	Clin Chem
resource representing a document's body	covid:f37db64e5c752c16ecb31debb4cbc5db69ba1969#body_text
is schema:about of	named entity 'Conclusion' named entity 'acid' named entity 'COVID-19' named entity 'viral' named entity 'sample' named entity 'specimens' named entity 'specimens' named entity 'acid' named entity 'PROTECTION' named entity 'FALSE NEGATIVES' named entity 'APPROACH' named entity 'TIMES' named entity 'TESTING' named entity 'QUANTITATIVE RT-PCR' named entity 'SARS-COV-2' named entity '282' named entity 'ABOUT' named entity 'MANAGEMENT' named entity 'VIRAL' named entity 'LOADS' named entity 'POTENTIAL' named entity 'RESULTS' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2' named entity 'quantitative' named entity 'NAT' named entity 'COVID-19' named entity 'false negatives' named entity 'NAT' named entity 'samples' named entity 'SARS-CoV-2' named entity 'polymerase' named entity 'limited' named entity 'Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)' named entity 'false-negative' named entity 'samples' named entity 'RT-PCR' named entity 'false-negative' named entity 'RT-PCR' named entity 'virus' named entity 'RT-PCR' named entity 'Corona Virus Disease' named entity 'real-time polymerase chain reaction' named entity 'COVID' named entity 'RT-PCR' named entity 'Severe Acute Respiratory Syndrome Coronavirus 2' named entity 'RT-PCR' named entity 'sputum' named entity 'viral RNA' named entity 'COVID' named entity 'ThermoFisher' named entity 'guanidinium' named entity 'MERS-CoV' named entity 'RT-PCR' named entity 'NAT' named entity 'throat swab' named entity 'false-negative' named entity 'RNA extraction' named entity 'sputum' named entity 'NAT' named entity 'La Jolla' named entity 'real-time PCR' named entity 'PCR' named entity 'SARS-CoV' named entity '60 minutes' named entity 'acute respiratory failure' named entity 'RT-PCR' named entity 'false-negative' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'clinical trials' named entity 'Severe Acute Respiratory Syndrome Coronavirus 2' named entity 'significant result' named entity '1, 2, 3, 4'

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