About: C. difficile 630Δerm Spo0A Regulates Sporulation, but Does Not Contribute to Toxin Production, by Direct High-Affinity Binding to Target DNA

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About: C. difficile 630Δerm Spo0A Regulates Sporulation, but Does Not Contribute to Toxin Production, by Direct High-Affinity Binding to Target DNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	C. difficile 630Δerm Spo0A Regulates Sporulation, but Does Not Contribute to Toxin Production, by Direct High-Affinity Binding to Target DNA
Creator	Bakker, Dennis Kuijper, Ed Rosenbusch, Katharina Smits, Klaas
Source	PMC
abstract	Clostridium difficile is a Gram positive, anaerobic bacterium that can form highly resistant endospores. The bacterium is the causative agent of C. difficile infection (CDI), for which the symptoms can range from a mild diarrhea to potentially fatal pseudomembranous colitis and toxic megacolon. Endospore formation in Firmicutes, including C. difficile, is governed by the key regulator for sporulation, Spo0A. In Bacillus subtilis, this transcription factor is also directly or indirectly involved in various other cellular processes. Here, we report that C. difficile Spo0A shows a high degree of similarity to the well characterized B. subtilis protein and recognizes a similar binding sequence. We find that the laboratory strain C. difficile 630Δerm contains an 18bp-duplication near the DNA-binding domain compared to its ancestral strain 630. In vitro binding assays using purified C-terminal DNA binding domain of the C. difficile Spo0A protein demonstrate direct binding to DNA upstream of spo0A and sigH, early sporulation genes and several other putative targets. In vitro binding assays suggest that the gene encoding the major clostridial toxin TcdB may be a direct target of Spo0A, but supernatant derived from a spo0A negative strain was no less toxic towards Vero cells than that obtained from a wild type strain, in contrast to previous reports. These results identify for the first time direct (putative) targets of the Spo0A protein in C. difficile and make a positive effect of Spo0A on production of the large clostridial toxins unlikely.
has issue date	2012-10-31(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0048608
bibo:pmid	23119071
has license	cc-by
sha1sum (hex)	f0fb3bbd96dad4c907c7fd456cd5783ed8fa7bd6
schema:url	https://doi.org/10.1371/journal.pone.0048608
resource representing a document's title	C. difficile 630Δerm Spo0A Regulates Sporulation, but Does Not Contribute to Toxin Production, by Direct High-Affinity Binding to Target DNA
has PubMed Central identifier	PMC3485338
has PubMed identifier	23119071
schema:publication	PLoS One
resource representing a document's body	covid:f0fb3bbd96dad4c907c7fd456cd5783ed8fa7bd6#body_text
is schema:about of	named entity 'diarrhea' named entity 'sporulation' named entity 'laboratory' named entity 'Toxin' named entity 'Not' covid:arg/f0fb3bbd96dad4c907c7fd456cd5783ed8fa7bd6 named entity 'similarity' named entity 'Bacillus' named entity 'protein' named entity 'genes' named entity 'C. difficile' named entity 'anaerobic' named entity 'demonstrate' named entity 'assays' named entity 'These' named entity 'DNA' named entity 'endospores' named entity 'B. subtilis' named entity 'causative' named entity 'High' named entity 'DNA' named entity 'Target' named entity 'C. difficile infection' named entity 'anaerobic bacterium' named entity 'toxin' named entity 'C-terminal' named entity 'B. subtilis' named entity 'supernatant' named entity 'diarrhea' named entity 'C. difficile' named entity 'laboratory strain' named entity 'cellular processes' named entity 'protein' named entity 'toxin' named entity 'transcription' named entity 'functional domains' named entity 'tcdR' named entity 'transition state' named entity 'homolog' named entity 'resuspension' named entity 'C. difficile' named entity 'phosphorylation' named entity 'C. difficile' named entity 'sporulation' named entity 'Novagen' named entity 'motif' named entity 'protein' named entity 'lipoate' named entity 'clostridial' named entity 'motif' named entity 'binding site' named entity 'C. difficile' named entity 'plasmid' named entity 'Southern blot' named entity 'promoter regions' named entity 'solvent' named entity 'penicillin' named entity 'DNA fragments' named entity 'sporulation' named entity 'sporulation' named entity 'pH 8' named entity 'CDI' named entity 'genome' named entity 'primers' named entity 'Sanger Institute' named entity 'Plasmid' named entity 'cloning' named entity 'protein' named entity 'supernatants' named entity 'binding site' named entity 'lysates' named entity 'stomach' named entity 'TcdB' named entity 'EMSA' named entity 'Firmicutes' named entity 'antibodies' named entity 'tcdB' named entity 'gene'

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