About: siRNAs targeting terminal sequences of the SARS-associated coronavirus membrane gene inhibit M protein expression through degradation of M mRNA

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About: siRNAs targeting terminal sequences of the SARS-associated coronavirus membrane gene inhibit M protein expression through degradation of M mRNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	siRNAs targeting terminal sequences of the SARS-associated coronavirus membrane gene inhibit M protein expression through degradation of M mRNA
Creator	Zhao, Ping Qi, Zhong-Tian Cao, Ming-Mei Qin, Zhao-Ling
source	Elsevier; Medline; PMC
abstract	Abstract SARS-associated coronavirus (SCoV) M protein plays a key role in viral assembly and budding. Recent studies revealed that M protein could interact with N protein in the Golgi complex. In this study, we showed that SCoV M protein co-localized in the Golgi apparatus with a Golgi vector marker. To study M protein function, three candidate small interfering RNAs (siRNAs) corresponding to M gene sequences were designed, transcribed in vitro, and then tested for their ability to silence M protein expression. The plasmid, pEGFP-M, encoding SCoV M protein as a fusion protein with EGFP, was used for silencing and for reporter gene detection in HEK 293T cells transfected with siRNA constructs. The results showed that the mean green fluorescence intensity and M RNA transcripts were significantly reduced, and that the expression of M glycoprotein was strongly inhibited in those cells co-transfected with M-specific siRNAs. These findings demonstrated that the three M-specific siRNAs were able to specifically and effectively inhibit M glycoprotein expression in cultured cells by blocking the accumulation of mRNA, which provides an approach for studies on the functions of M protein and for the development of novel prophylactic or therapeutic agents for SCoV infection.
has issue date	2007-11-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2007.05.017
bibo:pmid	17590445
has license	els-covid
sha1sum (hex)	f0b1ce7d32463fa6a36a47c7cf0e3fd6d48c0d6f
schema:url	https://doi.org/10.1016/j.jviromet.2007.05.017
resource representing a document's title	siRNAs targeting terminal sequences of the SARS-associated coronavirus membrane gene inhibit M protein expression through degradation of M mRNA
has PubMed Central identifier	PMC7112935
has PubMed identifier	17590445
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:f0b1ce7d32463fa6a36a47c7cf0e3fd6d48c0d6f#body_text
is schema:about of	named entity 'MRNA' named entity 'M protein' named entity 'Recent' named entity 'siRNAs' named entity 'expression' named entity 'accumulation' named entity 'fusion protein' named entity 'GREEN ' named entity 'FUSION PROTEIN' named entity 'INTERACT' named entity 'MEAN' named entity 'CULTURED CELLS' named entity 'SARS-ASSOCIATED CORONAVIRUS' named entity 'VECTOR' named entity 'GOLGI' named entity 'PROVIDES' named entity 'REDUCED' named entity 'SILENCE' named entity 'VIRAL ASSEMBLY' named entity 'BUDDING' named entity 'RECENT' named entity 'MRNA' named entity 'SMALL INTERFERING RNAS' named entity 'CANDIDATE' named entity 'SPECIFIC' named entity 'HEK' named entity 'SEQUENCES' named entity 'SIRNA' named entity 'M PROTEIN' named entity 'NOVEL' named entity 'LOCALIZED' named entity 'MARKER' named entity 'INFECTION' named entity 'THEIR' named entity 'APPROACH' named entity 'PLASMID' named entity 'FINDINGS' named entity 'THESE' named entity 'CELLS' named entity 'EXPRESSION' named entity 'DEVELOPMENT' named entity 'TARGETING' named entity 'FLUORESCENCE INTENSITY' named entity 'BLOCKING' named entity 'IN VITRO' named entity 'PROTEIN EXPRESSION' named entity 'M PROTEIN' named entity 'TRANSCRIPTS' named entity 'REPORTER GENE' named entity 'GOLGI COMPLEX' named entity 'N PROTEIN' named entity 'PROTEIN FUNCTION' named entity 'THERAPEUTIC AGENTS' named entity 'PLAYS' named entity 'DETECTION' named entity 'SIRNAS' named entity 'PROTEIN ' named entity 'INHIBITED' named entity 'PROTEIN EXPRESSION' named entity 'KEY' named entity '293T CELLS' named entity 'ACCUMULATION' named entity 'SARS-ASSOCIATED CORONAVIRUS' named entity 'SEQUENCES' named entity 'MEMBRANE' named entity 'TERMINAL' named entity 'SIRNAS' named entity 'DEGRADATION' named entity 'SILENCING' named entity 'REVEALED' named entity 'M GENE' named entity 'TRANSCRIBED' named entity 'STUDY' named entity 'ROLE' named entity 'DEMONSTRATED' named entity 'FUNCTIONS' named entity 'STUDIES' named entity 'GLYCOPROTEIN'

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