About: Development of a Rapid Automated Influenza A, Influenza B, and Respiratory Syncytial Virus A/B Multiplex Real-Time RT-PCR Assay and Its Use during the 2009 H1N1 Swine-Origin Influenza Virus Epidemic in Milwaukee, Wisconsin

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of a Rapid Automated Influenza A, Influenza B, and Respiratory Syncytial Virus A/B Multiplex Real-Time RT-PCR Assay and Its Use during the 2009 H1N1 Swine-Origin Influenza Virus Epidemic in Milwaukee, Wisconsin Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of a Rapid Automated Influenza A, Influenza B, and Respiratory Syncytial Virus A/B Multiplex Real-Time RT-PCR Assay and Its Use during the 2009 H1N1 Swine-Origin Influenza Virus Epidemic in Milwaukee, Wisconsin
Creator	Fan, Jiang He, Jie Kumar, Swati Darga, Patrick Patitucci, Teresa Wilkinson, Kimberly Beck, Eric Bose, Michael Henrickson, Kelly Jurgens, Lisa Kehl, Sue Lague, Elizabeth Witt, Lorraine
source	Elsevier; Medline; PMC
abstract	Rapid, semiautomated, and fully automated multiplex real-time RT-PCR assays were developed and validated for the detection of influenza (Flu) A, Flu B, and respiratory syncytial virus (RSV) from nasopharyngeal specimens. The assays can detect human H1N1, H3N2, and swine-origin (S-OIV) H1N1 Flu A viruses and were effectively used to distinguish Flu A infections (of all subtypes) from Flu B and RSV infections during the current S-OIV outbreak in Milwaukee, WI. The analytical limits of detection were 10(−2) to 10(1) TCID(50)/ml depending on the platform and analyte and showed only one minor cross-reaction among 23 common respiratory pathogens (intermittent cross-reaction to adenovirus at >10(7) TCID(50)/ml). A total of 100 clinical samples were tested by tissue culture, both automated assays, and the US Food and Drug Administration-approved ProFlu+ assay. Both the semiautomated and fully automated assays exhibited greater overall (Flu A, Flu B, and RSV combined) clinical sensitivities (93 and 96%, respectively) and individual Flu A sensitivities (100%) than the Food and Drug Administration-approved test (89% overall sensitivity and 93% Flu A sensitivity). All assays were 99% specific. During the S-OIV outbreak in Milwaukee, WI, the fully automated assay was used to test 1232 samples in 2 weeks. Flu A was detected in 134 clinical samples (126 H1N1 S-OIV, 5 H1N1 [human], and 1 untyped) with 100% positive agreement compared with other “in-house” validated molecular assays, with only 2 false-positive results. Such accurate testing using automated high-throughput molecule systems should allow clinicians and public health officials to react quickly and effectively during viral outbreaks.
has issue date	2010-01-01(xsd:dateTime)
bibo:doi	10.2353/jmoldx.2010.090095
bibo:pmid	19959800
has license	hybrid-oa
sha1sum (hex)	ef9426dc8a31adefc89171705782641cbe40ef35
schema:url	https://doi.org/10.2353/jmoldx.2010.090095
resource representing a document's title	Development of a Rapid Automated Influenza A, Influenza B, and Respiratory Syncytial Virus A/B Multiplex Real-Time RT-PCR Assay and Its Use during the 2009 H1N1 Swine-Origin Influenza Virus Epidemic in Milwaukee, Wisconsin
has PubMed Central identifier	PMC2797721
has PubMed identifier	19959800
schema:publication	The Journal of Molecular Diagnostics
resource representing a document's body	covid:ef9426dc8a31adefc89171705782641cbe40ef35#body_text
is schema:about of	named entity '2FB' named entity 'MULTIPLEX' named entity 'tissue culture' named entity 'specimens' named entity 'Flu' named entity 'infections' named entity 'H3N2' named entity 'influenza (Flu)' named entity 'Respiratory Syncytial Virus' named entity 'A/B' named entity 'Rapid' named entity 'H1N1 FLU' named entity 'HIGH' named entity 'INFECTIONS' named entity 'POSITIVE' named entity 'H1N1' named entity 'OVERALL' named entity 'ORIGIN' named entity 'TESTED' named entity 'VIRUSES' named entity 'MULTIPLEX' named entity 'CLINICIANS' named entity 'THROUGHPUT' named entity 'ACCURATE' named entity 'REAL-TIME RT-PCR' named entity 'SUBTYPES' named entity 'INFLUENZA' named entity 'PLATFORM' covid:arg/ef9426dc8a31adefc89171705782641cbe40ef35 named entity 'RT-PCR ASSAY' named entity 'WISCONSIN' named entity 'DETECT' named entity 'EPIDEMIC' named entity 'INFLUENZA B' named entity 'TEST' named entity 'COMPARED' named entity 'RSV' named entity 'ADENOVIRUS' named entity 'H3N2' named entity 'COMBINED' named entity '28126' named entity 'ALLOW' named entity 'DEVELOPMENT' named entity 'INFLUENZA A' named entity 'ORIGIN' named entity 'H1N1' named entity 'SWINE' named entity 'RESPIRATORY SYNCYTIAL VIRUS A' named entity 'RAPID' named entity 'ITS' named entity 'USE' named entity 'INFLUENZA VIRUS' named entity 'AUTOMATED' named entity 'SWINE' named entity 'VALIDATED' named entity 'VIRAL' named entity 'SYSTEMS' named entity 'MILWAUKEE' named entity '100%' named entity 'SEMIAUTOMATED' named entity 'GREATER' named entity 'DETECTION' named entity 'RAPID' named entity 'ANALYTICAL' named entity 'LIMITS OF DETECTION' named entity 'REACT' named entity '134' named entity 'SENSITIVITY' named entity 'TESTING'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software