About: Development of an ELISA-array for simultaneous detection of five encephalitis viruses

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About: Development of an ELISA-array for simultaneous detection of five encephalitis viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of an ELISA-array for simultaneous detection of five encephalitis viruses
Creator	Liu, Hong Li, Jing Hu, Yi Yang, Yinhui Lin, Fang Chang, Guohui Zhu, Qingyu Fan, Li Kang, Xiaoping Li, Yuchang Wei, Jingjing Zhu, Xiaolei
Source	Medline; PMC
abstract	Japanese encephalitis virus(JEV), tick-borne encephalitis virus(TBEV), and eastern equine encephalitis virus (EEEV) can cause symptoms of encephalitis. Establishment of accurate and easy methods by which to detect these viruses is essential for the prevention and treatment of associated infectious diseases. Currently, there are still no multiple antigen detection methods available clinically. An ELISA-array, which detects multiple antigens, is easy to handle, and inexpensive, has enormous potential in pathogen detection. An ELISA-array method for the simultaneous detection of five encephalitis viruses was developed in this study. Seven monoclonal antibodies against five encephalitis-associated viruses were prepared and used for development of the ELISA-array. The ELISA-array assay is based on a %22sandwich%22 ELISA format and consists of viral antibodies printed directly on 96-well microtiter plates, allowing for direct detection of 5 viruses. The developed ELISA-array proved to have similar specificity and higher sensitivity compared with the conventional ELISAs. This method was validated by different viral cultures and three chicken eggs inoculated with infected patient serum. The results demonstrated that the developed ELISA-array is sensitive and easy to use, which would have potential for clinical use.
has issue date	2012-02-27(xsd:dateTime)
bibo:doi	10.1186/1743-422x-9-56
bibo:pmid	22369052
has license	cc-by
sha1sum (hex)	ef2b8f83d5a3ab8ae35e4b51fea6d3ed9eb49122
schema:url	https://doi.org/10.1186/1743-422x-9-56
resource representing a document's title	Development of an ELISA-array for simultaneous detection of five encephalitis viruses
has PubMed Central identifier	PMC3305475
has PubMed identifier	22369052
schema:publication	Virol J
resource representing a document's body	covid:ef2b8f83d5a3ab8ae35e4b51fea6d3ed9eb49122#body_text
is schema:about of	named entity 'essential' named entity 'Seven' named entity 'format' named entity 'viruses' named entity 'HAVE' named entity 'ELISA' named entity 'METHODS' named entity 'VIRUSES' named entity 'SENSITIVITY' named entity 'INFECTIOUS DISEASES' named entity 'DIRECT DETECTION' named entity 'USED' named entity 'PREVENTION' named entity 'COMPARED' named entity 'DETECTION' named entity 'PRINTED' named entity 'TO DETECT' named entity 'ENCEPHALITIS VIRUSES' named entity 'BASED' named entity 'DIFFERENT' named entity 'STUDY' named entity 'CURRENTLY' named entity 'ESTABLISHMENT' named entity 'VIRAL' named entity 'THESE' named entity 'HANDLE' named entity 'ASSOCIATED' named entity 'CLINICAL' named entity 'CULTURES' named entity 'ANTIGEN DETECTION' named entity 'ACCURATE' named entity 'PLATES' named entity 'MULTIPLE' named entity 'EASY' named entity 'DEMONSTRATED' named entity 'MONOCLONAL ANTIBODIES' named entity 'ARRAY ASSAY' named entity 'VIRAL ANTIBODIES' named entity 'INFECTED' named entity 'SENSITIVE' named entity 'TICK-BORNE ENCEPHALITIS VIRUS' named entity 'SERUM' named entity 'CONVENTIONAL' named entity 'DEVELOPMENT' named entity 'ALLOWING' covid:arg/ef2b8f83d5a3ab8ae35e4b51fea6d3ed9eb49122 named entity 'SPECIFICITY' named entity 'ANTIGENS' named entity 'EASTERN EQUINE ENCEPHALITIS VIRUS' named entity 'EGGS' named entity 'SIMULTANEOUS' named entity 'ELISA' named entity 'DEVELOPMENT' named entity 'SIMILAR' named entity 'DETECTION' named entity 'USE' named entity 'CHICKEN' named entity 'DETECTION METHODS' named entity 'HIGHER' named entity 'AVAILABLE' named entity 'PREPARED' named entity 'VALIDATED' named entity 'METHOD' named entity 'DIRECTLY' named entity 'FORMAT' named entity 'SIMULTANEOUS' named entity 'RESULTS' named entity 'ESSENTIAL' named entity 'ENCEPHALITIS VIRUSES' named entity 'ARRAY'

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