About: Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus

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About: Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus
Creator	Wang, Wei Gao, Xiao-Ming Li, Yonghua Ren, Furong Wang, Guang-Fa Xu, Xiaoyuan Zhao, Jincun Zhao, Zhendong Zhuang, Hui
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Severe acute respiratory syndrome (SARS) is caused by infection with SARS-associated coronavirus (CoV). Amino acid residues 450–650 of the spike (S) glycoprotein of SARS-CoV (S450-650) contains dominant epitopes for anti-viral antibodies (Abs) in patient sera. OBJECTIVES: To develop and evaluate an ELISA system for detection of anti-S Abs in patient sera. STUDY DESIGN: Express recombinant S450-650 in E. Coli and evaluate the sensitivity and specificity of an ELISA system based on the S450-650 polypeptide. RESULTS: The S450-650-based ELISA detected IgG Abs in 41 out of 51 serum samples from 22 hospitalized patients with probable SARS, a result closely correlated with that obtained with a virus-based ELISA (r = 0.75, k = 0.8). Differential anti-S IgG responses were observed amongst SARS patients. Some of them produced anti-S Abs early during their infection, while others failed to make IgG Abs against the S450-650 polypeptide. None of the serum samples from 100 healthy blood donors was positive in the S450-650-based assay. CONCLUSION: The S450-650-based ELISA can detect anti-S IgG Abs with high sensitivity and specificity.
has issue date	2004-11-23(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2004.09.024
bibo:pmid	15797360
has license	no-cc
sha1sum (hex)	ef1852fa8f6d2f389be7f146b3374c2d33ea1b81
schema:url	https://doi.org/10.1016/j.jcv.2004.09.024
resource representing a document's title	Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus
has PubMed Central identifier	PMC7108335
has PubMed identifier	15797360
schema:publication	J Clin Virol
resource representing a document's body	covid:ef1852fa8f6d2f389be7f146b3374c2d33ea1b81#body_text
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