About: Heterogeneity of infectious bronchitis virus grown in eggs

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Heterogeneity of infectious bronchitis virus grown in eggs
Creator	Alexander, D Collins I, M Hai~ks, J
Source	PMC
abstract	Egg-grown infectious bronchitis virus, strain Beaudette, was concentrated and centrifuged on sucrose density gradients to separate the virus into five peaks with densities of 1.144, 1.160, 1.172, 1.191 and 1.218 g/cm(3). All peaks retained infectivity, complement fixation activity and were labelled with(3)H-uridine. Morphologically the densest peak consisted of very large virus particles and amorphous material, the other peaks consisted of mainly intact particles although small differences in size and pleomorphism were seen. Polyacrylamide gel electrophoresis of material from the density gradient peaks revealed four major polypeptides and at least 10 minor polypeptides. The proportions of the polypeptides were approximately similar for all peaks with the exception of the densest peak in which the major polypeptides were greatly reduced. The four major polypeptides had approximate molecular weights of 1. 52,000, 2. 45,000, 3. 34,000, 4. 32,000. The major polypeptides 1 and 4 were shown to be glycosylated as were two of the minor polypeptides.
has issue date	1976-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01318001
bibo:pmid	176969
has license	no-cc
sha1sum (hex)	eecf968682082e196999faa0787c0492ac1d0c35
schema:url	https://doi.org/10.1007/bf01318001
resource representing a document's title	Heterogeneity of infectious bronchitis virus grown in eggs
has PubMed Central identifier	PMC7086894
has PubMed identifier	176969
schema:publication	Arch Virol
resource representing a document's body	covid:eecf968682082e196999faa0787c0492ac1d0c35#body_text
is schema:about of	named entity 'concentrated' named entity 'peaks' named entity 'PEAK' named entity 'VIRUS PARTICLES' named entity 'EGG' named entity 'STRAIN' named entity 'AMORPHOUS' named entity 'INTACT' named entity 'DENSITY' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'CONCENTRATED' named entity 'ACTIVITY' named entity 'RETAINED' named entity 'GRADIENTS' named entity 'PLEOMORPHISM' named entity 'COMPLEMENT FIXATION' named entity 'EGGS' named entity 'TO SEPARATE' named entity 'SUCROSE' named entity '160' named entity 'INFECTIVITY' named entity '172' named entity 'VIRUS' named entity '144' named entity 'URIDINE' named entity 'SMALL' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'SEEN' named entity 'HETEROGENEITY' named entity 'PEAKS' named entity 'VERY LARGE' named entity 'CENTRIFUGED' named entity 'SIZE' named entity '191' named entity 'PARTICLES' named entity 'DENSITIES' named entity '218' named entity 'MATERIAL' covid:arg/eecf968682082e196999faa0787c0492ac1d0c35 named entity 'virus' named entity 'virus' named entity 'size' named entity 'virus' named entity 'complement fixation' named entity 'retained' named entity 'All peaks' named entity 'densities' named entity 'Bronchitis' named entity 'density gradients' named entity 'infectious bronchitis virus' named entity 'Morphologically' named entity 'virus' named entity 'infectivity' named entity 'complement fixation' named entity 'sucrose' named entity 'Sendai virus' named entity 'uridine' named entity 'morphogenesis' named entity 'buoyant density' named entity 'virus' named entity 'ALS' named entity 'sucrose' named entity 'human embryo' named entity 'paramyxoviruses' named entity 'electron microscopic' named entity 'unit cell' named entity 'IBV' named entity 'polypeptide' named entity 'high molecular weight' named entity 'virus' named entity 'virus' named entity 'organ' named entity 'IBV' named entity 'virus' named entity '40 minutes' named entity 'virus' named entity 'sucrose' named entity 'infectivity' named entity 'antisera'

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