About: Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome

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About: Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome
Creator	Bradley, Daniel Branch, Hepatitis Fry, Kirk Guerra, Martha Huang, Chiao-Chain Reyes', Gregory Smith, Matthew Tam, Albert
source	Elsevier; Medline; PMC
abstract	Abstract We have recently described the cloning of a portion of the hepatitis E virus (HEV) and confirmed its etiologic association with enterically transmitted (waterborne, epidemic) non-A, non-B hepatitis. The virus consists of a single-stranded, positive-sense RNA genome of approximately 7.5 kb, with a polyadenylated 3' end. We now report on the cloning and nucleotide sequencing of an overlapping, contiguous set of cDNA clones representing the entire genome of the HEV Burma strain [HEV(B)]. The largest open reading frame extends approximately 5 kb from the Fend and contains the RNA-directed RNA polymerase and nucleoside triphosphate binding motifs. The second major open reading frame (ORF2) begins 37 by downstream of the first and extends approximately 2 kb to the termination codon present 65 by from the 3' terminal stretch of poly(A) residues. ORF2 contains a consensus signal peptide sequence at its amino terminus and a capsid-like region with a high content of basic amino acids similar to that seen with other virus capsid proteins. A third open reading frame partially overlaps the first and second and encompasses only 369 bp. In addition to the 7.5-kb full-length genomic transcript, two subgenomic polyadenylated messages of approximately 3.7 and 2.0 kb were detected in infected liver using a probe from the 3' third of the genome. The genomic organization of the virus is consistent with the Fend encoding nonstructural and the 3' end encoding the viral structural gene(s). The expression strategy of the virus involves the use of three different open reading frames and at least three different transcripts. HEV was previously determined to be a nonenveloped particle with a diameter of 27–34 nm. These findings on the genetic organization and expression strategy of HEV suggest that it is the prototype human pathogen for a new class of RNA virus or perhaps a separate genus within the Caliciviridae family
has issue date	1991-11-30(xsd:dateTime)
bibo:doi	10.1016/0042-6822(91)90760-9
bibo:pmid	1926770
has license	els-covid
sha1sum (hex)	ee920d9fbd665ab4530646653d2ca966f109e333
schema:url	https://doi.org/10.1016/0042-6822%2891%2990760-9
resource representing a document's title	Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome
has PubMed Central identifier	PMC7130833
has PubMed identifier	1926770
schema:publication	Virology
resource representing a document's body	covid:ee920d9fbd665ab4530646653d2ca966f109e333#body_text
is schema:about of	named entity 'HEPATITIS E VIRUS' named entity 'representing' named entity 'bp.' named entity 'cDNA clones' named entity 'transcript' named entity 'etiologic' named entity 'virus' named entity 'set' named entity 'strategy' named entity 'basic' named entity 'POSITIVE' named entity 'LENGTH' named entity 'TERMINAL' named entity 'TRANSMITTED' named entity 'SET' named entity 'PATHOGEN' named entity '7.5' named entity 'STRATEGY' named entity 'HUMAN' named entity 'RNA-DIRECTED RNA POLYMERASE' named entity 'CDNA' named entity 'EXPRESSION' named entity 'REPORT' named entity 'ITS' named entity 'PROBE' named entity '28S' named entity 'BURMA' named entity 'FINDINGS' named entity 'ENTIRE' named entity 'LIVER' named entity 'OVERLAPPING' named entity 'CONSENSUS' named entity 'CLONES' named entity 'B HEPATITIS' named entity 'MESSAGES' named entity 'SENSE' named entity 'CONTIGUOUS' named entity 'USING' named entity 'HEV' named entity 'PRESENT' named entity 'PARTIALLY' named entity 'CLONING' named entity 'DOWNSTREAM' named entity 'SUGGEST' named entity 'LARGEST' named entity 'OVERLAPS' named entity 'GENETIC' named entity 'MAJOR' named entity '28A' named entity 'VIRAL STRUCTURAL GENE' named entity 'STRETCH' named entity 'HIGH' named entity 'ACADEMIC' covid:arg/ee920d9fbd665ab4530646653d2ca966f109e333 named entity 'APPROXIMATELY' named entity 'CONFIRMED' named entity 'TRANSCRIPT' named entity 'POLY' named entity 'CLASS' named entity 'NON-' named entity 'SECOND' named entity 'CONTAINS' named entity 'BEGINS' named entity 'AMINO' named entity 'ASSOCIATION' named entity 'SEEN' named entity 'END' named entity 'NUCLEOTIDE SEQUENCING' named entity 'ENCODING' named entity 'POLYADENYLATED' named entity 'SIMILAR' named entity 'NUCLEOSIDE TRIPHOSPHATE' named entity 'TERMINATION CODON' named entity 'TERMINUS'

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