About: Evaluation of 793/B-like and Mass-like vaccine strain kinetics in experimental and field conditions by real-time RT-PCR quantification

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About: Evaluation of 793/B-like and Mass-like vaccine strain kinetics in experimental and field conditions by real-time RT-PCR quantification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of 793/B-like and Mass-like vaccine strain kinetics in experimental and field conditions by real-time RT-PCR quantification
Creator	Catelli, E Cecchinato, M Berto, G Drigo, M Franzo, G Koutoulis, K Ramon, G Tucciarone, C
source	Medline; PMC
abstract	Infectious bronchitis virus (IBV) is a great economic burden both for productive losses and costs of the control strategies. Many different vaccination protocols are applied in the same region and even in consecutive cycles on the same farm in order to find the perfect balance between costs and benefits. In Northern Italy, the usual second vaccination is more and more often moved up to the chick's first d of life. The second strain administration together with the common Mass priming by spray at the hatchery allows saving money and time and reducing animal stress. The present work compared the different vaccine strains (Mass-like or B48, and 1/96) kinetics both in field conditions and in a 21-day-long experimental trial in broilers, monitoring the viral replication by upper respiratory tract swabbing and vaccine specific real time reverse transcription PCR (RT-PCR) quantification. In both field and experimental conditions, titers for all the vaccines showed an increasing trend in the first 2 wk and then a decrease, though still remaining detectable during the whole monitored period. IBV field strain and avian Metapneumovirus (aMPV) presence also was also investigated by RT-PCR and sequencing, and by multiplex real-time RT-PCR, respectively, revealing a consistency in the pathogen introduction timing at around 30 d, in correspondence with the vaccine titer's main decrease. These findings suggest the need for an accurate knowledge of live vaccine kinetics, whose replication can compete with the other pathogen one, providing additional protection to be added to what is conferred by the adaptive immune response.
has issue date	2017-10-25(xsd:dateTime)
bibo:doi	10.3382/ps/pex292
bibo:pmid	29077954
has license	no-cc
sha1sum (hex)	ee17beb088764da5cee76084ffb093fb4b4ff396
schema:url	https://doi.org/10.3382/ps/pex292
resource representing a document's title	Evaluation of 793/B-like and Mass-like vaccine strain kinetics in experimental and field conditions by real-time RT-PCR quantification
has PubMed Central identifier	PMC7107209
has PubMed identifier	29077954
schema:publication	Poult Sci
resource representing a document's body	covid:ee17beb088764da5cee76084ffb093fb4b4ff396#body_text
is schema:about of	named entity 'field' named entity 'stress' named entity 'farm' named entity 'period' named entity 'experimental' named entity 'VACCINE STRAIN' named entity 'EXPERIMENTAL' named entity 'TIMING' named entity 'EXPERIMENTAL TRIAL' named entity 'FIELD' named entity 'CONTROL' named entity 'PRIMING' named entity 'COMPARED' named entity 'TIME' named entity 'B48' named entity 'ADMINISTRATION' named entity 'WORK' named entity 'KINETICS' named entity 'MORE OFTEN' named entity 'PRESENCE' named entity '27S' named entity 'ADDITIONAL' named entity 'ACCURATE' named entity 'MONITORING' named entity 'DIFFERENT' named entity 'MONEY' named entity 'FIELD' named entity 'CONDITIONS' named entity 'REPLICATION' named entity 'VIRAL REPLICATION' named entity 'PRODUCTIVE' named entity 'SECOND' named entity 'SPECIFIC' named entity 'COMMON' named entity 'ANIMAL' named entity 'USUAL' named entity 'AVIAN METAPNEUMOVIRUS' named entity 'CONSISTENCY' named entity 'UPPER RESPIRATORY TRACT' named entity 'STRATEGIES' named entity 'GREAT' named entity 'QUANTIFICATION' named entity 'REGION' named entity 'ECONOMIC BURDEN' named entity 'PROTOCOLS' named entity 'DETECTABLE' named entity 'STRESS' named entity 'PROTECTION' named entity 'CONSECUTIVE' named entity 'KNOWLEDGE' named entity 'STRAIN' named entity 'REMAINING' named entity 'PERIOD' named entity 'EVALUATION' named entity 'KINETICS' named entity 'FARM' named entity 'INTRODUCTION' named entity 'IS A' named entity 'STRAINS' named entity 'ADDED' named entity 'INCREASING' named entity 'PROVIDING' named entity 'REVERSE TRANSCRIPTION PCR' named entity 'TREND' named entity 'MASS' named entity 'CONDITIONS' named entity '2FB' named entity 'COSTS' named entity 'ITALY' named entity 'PRESENT' named entity 'VACCINES' named entity 'REAL-TIME RT-PCR' named entity 'LIKE' named entity 'QUANTIFICATION'

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