About: Rice dwarf phytoreovirus segment S12 transcript is tricistronic in Vitro

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rice dwarf phytoreovirus segment S12 transcript is tricistronic in Vitro
Creator	Kusano, Tomonobu Sugawara, Mikiko Suzuki,', Nobuhiro
Source	Elsevier; Medline; PMC
abstract	Abstract Sequence analysis revealed that rice dwarf phytoreovirus segment S12 is 1066 nucleotides long with a small out-of-phase, overlapping open reading frame (ORF) as well as a major ORF. The large ORF (positions 42 to 980) encodes 312 amino acids, while the small one (bases 313 to 591) encodes 92 amino acids with an additional in-frame AUG codon (positions 337–339) 24 nucleotides downstream from the first one. Transcripts from a full-length cDNA directed the in vitro synthesis of three polypeptides of 33 (considered to be translated from the long ORF), 8, and 7 kDa. Alteration of each of the two ATG codons on the small ORF demonstrated their involvement in the generation of the 8- and 7-kDa polypeptides. Although it is still unknown whether these proteins are expressed in vivo, the small ORF is shown to be conserved in S9s of two other members of the genus Phytoreovirus, rice gall dwarf virus and wound tumor virus, suggesting its common, important function.
has issue date	1992-12-31(xsd:dateTime)
bibo:doi	10.1016/0042-6822(92)90279-x
bibo:pmid	1448932
has license	els-covid
sha1sum (hex)	ed2b5eeb0f31b0d75688d28dfbc4ae75bff7f273
schema:url	https://doi.org/10.1016/0042-6822%2892%2990279-x
resource representing a document's title	Rice dwarf phytoreovirus segment S12 transcript is tricistronic in Vitro
has PubMed Central identifier	PMC7130740
has PubMed identifier	1448932
schema:publication	Virology
resource representing a document's body	covid:ed2b5eeb0f31b0d75688d28dfbc4ae75bff7f273#body_text
is schema:about of	named entity 'CDNA' named entity 'SEGMENT' named entity 'TRANSCRIPTS' named entity '313' named entity 'OPEN READING FRAME' named entity 'TRANSLATED' named entity 'POLYPEPTIDES' named entity 'CODON' named entity 'IN VITRO' named entity 'SEGMENT' named entity 'ORF' named entity 'OF EACH' named entity 'OVERLAPPING' named entity '591' named entity 'GENERATION' named entity 'AMINO ACIDS' named entity 'TRANSCRIPT' named entity 'ADDITIONAL' named entity 'ALTERATION' named entity 'LONG' named entity 'RICE DWARF PHYTOREOVIRUS' named entity 'S12' named entity 'RICE DWARF PHYTOREOVIRUS' named entity 'DOWNSTREAM' named entity 'REVEALED' named entity 'VIM' named entity 'LARGE' named entity 'THEIR' named entity '312' named entity 'KDA' named entity 'A MAJOR' named entity 'ATG' named entity 'SEQUENCE ANALYSIS' named entity 'AUG' named entity 'SYNTHESIS' named entity '960' named entity 'S12' named entity 'DEMONSTRATED' named entity 'SMALL' named entity 'NUCLEOTIDES' named entity 'SMALL ORF' named entity 'DIRECTED' named entity 'LENGTH' named entity 'FRAME' named entity 'INVOLVEMENT' named entity 'polypeptides' named entity 'Sequence analysis' named entity 'ORF' named entity 'polypeptides' named entity 'in-frame' named entity 'nucleotides' named entity 'phytoreovirus' named entity 'cDNA' named entity 'amino acids' named entity 'ORF' named entity 'Phytoreovirus' named entity 'Vim' named entity 'C/al' named entity 'RDV' named entity 'mRNAs' named entity 'RDV' named entity 'PCR' named entity 'WlV' named entity 'mRNAs' named entity 'cDNAs' named entity 'rice' named entity 'pUC' named entity 'Koganezawa' named entity 'fluorography' named entity 'site-directed' named entity 'life cycle' named entity 'amino acids' named entity 'primer' named entity 'ORF' named entity 'genus' named entity 'covering bases' named entity 'virus' named entity 'amino acids' named entity 'ORF'

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