About: RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot

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About: RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
Creator	Britton, Paul Cavanagh, Dave Culver, Francesca
Source	Medline; PMC
abstract	Of the many primer combinations that we have investigated for the detection of avian coronaviruses, two have worked better than any of the others: they worked with the largest number of strains/samples of a given coronavirus and the most species of avian coronavirus, and they also produced the most sensitive detection tests. The primer combinations were: oligonucleotide pair 2Bp/4Bm, which is in a region of gene 1 that is moderately conserved among all species of coronavirus (1); and UTR11-/UTR41+, which are in a highly conserved part of the 3′ untranslated region of avian coronaviruses related to infectious bronchitis virus (2). The gene 1 primer pair enabled the detection of a new coronavirus in a green-cheeked Amazon parrot (Amazon viridigenalis Cassin). In this chapter we describe the use of these oligonucleotides in a one-step (single-tube) RT-PCR, and describe the procedure that we used to extract RNA from turkey feces.
has issue date	2007-11-28(xsd:dateTime)
bibo:doi	10.1007/978-1-59745-181-9_4
bibo:pmid	19057865
has license	no-cc
sha1sum (hex)	ec8fb5fa275bebc7750f34837f1b6ea10edd21ae
schema:url	https://doi.org/10.1007/978-1-59745-181-9_4
resource representing a document's title	RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
has PubMed Central identifier	PMC7120991
has PubMed identifier	19057865
schema:publication	SARS- and Other Coronaviruses
resource representing a document's body	covid:ec8fb5fa275bebc7750f34837f1b6ea10edd21ae#body_text
is schema:about of	named entity 'RT-PCR' named entity 'species' named entity 'THESE' named entity 'SPECIES' named entity 'HAVE' named entity 'DETECTION' named entity 'STEP' named entity 'NEW' named entity 'AMAZON PARROT' named entity 'AVIAN CORONAVIRUS' named entity 'AVIAN CORONAVIRUSES' named entity 'RT-PCR' named entity 'GREEN-CHEEKED AMAZON' named entity 'HIGHLY' named entity 'OLIGONUCLEOTIDES' named entity 'NUMBER OF' named entity 'PAIR' named entity 'SENSITIVE' named entity 'PROCEDURE' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'RELATED' named entity 'STRAINS' named entity 'FECES' named entity 'TO EXTRACT' named entity 'PHEASANT' named entity 'MODERATELY' named entity 'UNTRANSLATED REGION' named entity 'TESTS' named entity 'GIVEN' named entity 'BETTER' named entity 'LARGEST' named entity 'PART OF' named entity 'USE OF' named entity 'COMBINATIONS' named entity 'CONSERVED' named entity 'BIRDS' named entity 'DETECTION' named entity 'PARROT' named entity 'TURKEY' named entity '282' named entity 'INVESTIGATED' named entity 'ENABLED' named entity 'USED' named entity 'RNA' named entity 'WORKED' named entity 'CORONAVIRUS' named entity 'TUBE' named entity 'REGION' named entity 'OLIGONUCLEOTIDE' named entity 'PRIMER' named entity 'DESCRIBE' named entity 'AVIAN CORONAVIRUSES' named entity 'TURKEY' named entity 'CHAPTER' named entity 'produced' named entity 'oligonucleotides' named entity 'investigated' named entity 'coronaviruses' named entity 'strains' named entity 'coronavirus' named entity 'Parrot' named entity 'Pheasant' named entity 'oligonucleotide' named entity 'coronavirus' named entity 'primer' named entity 'coronavirus' named entity 'extract RNA' named entity 'oligonucleotides' named entity 'coronaviruses' named entity 'Turkey' named entity 'Coronaviruses' named entity 'IBV' named entity 'gene' named entity '1.2' named entity 'coronaviruses' named entity 'thermocycler' named entity 'supernatant' named entity 'mallard duck' named entity 'pathogen'

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