About: Evaluation of Transport Media and Specimen Transport Conditions for the Detection of SARS-CoV-2 by Use of Real-Time Reverse Transcription-PCR

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of Transport Media and Specimen Transport Conditions for the Detection of SARS-CoV-2 by Use of Real-Time Reverse Transcription-PCR
Creator	Marlowe, Elizabeth Batterman, Hollis Baumann, Russell Borillo, Gwynngelle Galdzicka, Marzena Kagan, Ron Rogers, Amy
topic	covid:ec08809d467190884c3c3346e9bd7f88eaf8a128#this
source	Medline; PMC
abstract	The global coronavirus (CoV) disease 2019 (COVID-19) pandemic has resulted in a worldwide shortage of viral transport media and raised questions about specimen stability. The objective of this study was to determine the stability of severe acute respiratory syndrome CoV 2 (SARS-CoV-2) RNA in specimen transport media under various storage conditions. Transport media tested included UTM, UTM-RT, ESwab, M4, and saline (0.9% NaCl). Specimen types tested included nasopharyngeal/oropharyngeal swabs in the above-named transport media, bronchoalveolar lavage (BAL) fluid, and sputum. A high-titer SARS-CoV-2 remnant patient specimen was spiked into pooled SARS-CoV-2 RNA-negative specimen remnants for the various medium types. Aliquots of samples were stored at 18°C to 26°C, 2°C to 8°C, and −10°C to −30°C and then tested at time points up to 14 days. Specimens consistently yielded amplifiable RNA with mean cycle threshold differences of <3 over the various conditions assayed, thus supporting the use and transport of alternative collection media and specimen types under a variety of temperature storage conditions.
has issue date	2020-07-23(xsd:dateTime)
bibo:doi	10.1128/jcm.00708-20
bibo:pmid	32341141
has license	cc-by
sha1sum (hex)	ec08809d467190884c3c3346e9bd7f88eaf8a128
schema:url	https://doi.org/10.1128/jcm.00708-20
resource representing a document's title	Evaluation of Transport Media and Specimen Transport Conditions for the Detection of SARS-CoV-2 by Use of Real-Time Reverse Transcription-PCR
has PubMed Central identifier	PMC7383526
has PubMed identifier	32341141
schema:publication	J Clin Microbiol
resource representing a document's body	covid:ec08809d467190884c3c3346e9bd7f88eaf8a128#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/ec08809d467190884c3c3346e9bd7f88eaf8a128
is schema:about of	named entity 'spiked' named entity 'patient' named entity 'coronavirus' named entity 'Specimen' named entity 'Use' named entity 'BRONCHOALVEOLAR LAVAGE' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'CORONAVIRUS' named entity 'CONDITIONS' named entity 'TIME' named entity 'MEDIUM' named entity 'POOLED' named entity '2019' named entity 'specimen' named entity 'Specimens' named entity 'study' named entity 'severe acute respiratory syndrome' named entity 'SARS-CoV-2' named entity 'oropharyngeal' named entity 'sputum' named entity 'RNA' named entity 'severe acute respiratory syndrome' named entity 'bronchoalveolar lavage' named entity 'SARS-CoV-2' named entity 'Transport Media' named entity 'SARS-CoV-2' named entity 'nasopharyngeal' named entity 'World Health Organization' named entity 'SARS-CoV-2' named entity 'emergency use authorization' named entity 'titer' named entity 'Italy' named entity 'SARS-CoV-2' named entity 'oropharyngeal' named entity 'human coronaviruses' named entity 'COVID-19 pandemic' named entity 'WHO' named entity 'respiratory disease' named entity 'Infection' named entity 'viral RNA' named entity 'COVID' named entity 'NL63' named entity 'PCR' named entity 'PBS' named entity 'rRT-PCR' named entity 'VTM' named entity 'clinical testing' named entity 'SARS-CoV-2' named entity 'Infectious Disease' named entity 'aerosols' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'normal saline' named entity 'FDA' named entity 'nasopharyngeal' named entity 'RNA' named entity 'RNA' named entity 'adenovirus' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'herpes simplex virus' named entity 'Italy' named entity 'RNA' named entity 'titer' named entity 'sars-cov-2' named entity 'reverse transcription' named entity 'bronchoalveolar lavage' named entity '11 March 2020' named entity 'Quest Diagnostics' named entity 'virus' named entity 'EUA' named entity 'sputum' named entity 'viruses'

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