About: Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus
Creator	Avadhanula, Vasanthi Peret, Teresa Wang, Lijuan Machablishvili, Ann Durigon, Edison Piedra, Pedro Thornburg, Natalie López, María-Renée
Source	Elsevier; Medline; PMC
abstract	Abstract Human respiratory syncytial virus (HRSV) is a leading cause of acute respiratory illness in young children worldwide. Reliable detection and identification of HRSV subgroup A and B infections are essential for accurate disease burden estimates in anticipation of licensure of novel HRSV vaccines and immunotherapies. To ensure continued reliability, molecular assays must remain current with evolving virus strains. We have developed a HRSV subgroup-specific real-time RT-PCR (rRT-PCR) assay for detection and subgroup identification using primers and subgroup-specific probes targeting a conserved region of the nucleoprotein gene combined in a single duplex reaction using all genome sequence data currently available in GenBank. The assay was validated for analytical sensitivity, specificity, reproducibility, and clinical performance with a geographically diverse collection of viral isolates and respiratory specimens in direct comparison with an established pan-HRSV rRT-PCR reference test. The assay was sensitive, reproducibly detecting as few as 5–10 copies/reaction of target RNA. The assay was specific, showing no amplification with a panel of 16 other common respiratory pathogens or predicted by in silico primer/probe analysis. The duplex rRT-PCR assay based on the most current available genome sequence data permits rapid, sensitive and specific detection and subgroup identification of HRSV.
has issue date	2019-09-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2019.113676
bibo:pmid	31181218
has license	els-covid
sha1sum (hex)	eb1471a925db7e8edce579db5a5a3b4ee8372459
schema:url	https://doi.org/10.1016/j.jviromet.2019.113676
resource representing a document's title	Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus
has PubMed Central identifier	PMC7172218
has PubMed identifier	31181218
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:eb1471a925db7e8edce579db5a5a3b4ee8372459#body_text
is schema:about of	named entity 'panel' named entity 'evolving' named entity 'sequence' named entity 'virus' named entity 'nucleoprotein' named entity 'collection' named entity 'validated' named entity 'RT-PCR' named entity 'COMMON' named entity 'CURRENTLY' named entity 'PCR' named entity 'REACTION' named entity 'VALIDATED' named entity 'CURRENT' named entity 'WORLDWIDE' named entity 'ANALYTICAL' named entity 'CAUSE' named entity 'REAL-TIME RT-PCR' named entity 'PRIMERS' named entity 'GENBANK' named entity 'REPRODUCIBILITY' named entity 'COMPARISON' named entity 'TARGET' named entity 'ESSENTIAL' covid:arg/eb1471a925db7e8edce579db5a5a3b4ee8372459 named entity 'estimates' named entity 'detection' named entity 'vaccines' named entity 'assay' named entity 'worldwide' named entity 'gene' named entity 'reaction' named entity 'strains' named entity 'probe' named entity 'primers' named entity 'viral' named entity 'The duplex' named entity 'identification' named entity 'developed' named entity 'rRT-PCR' named entity 'rRT-PCR' named entity 'nucleoprotein' named entity 'genome sequence data' named entity 'Human respiratory syncytial virus' named entity 'real-time RT-PCR' named entity 'pathogen' named entity 'homology' named entity '5′-end' named entity 'coronaviruses' named entity 'HRSV' named entity 'HRSV' named entity 'HRSV' named entity 'annealing' named entity 'young children' named entity 'virus strain' named entity 'young children' named entity 'rRT-PCR' named entity 'HRSV' named entity 'informed consent' named entity 'pathogen' named entity 'HRSV' named entity 'rRT-PCR' named entity 'disease burden' named entity 'HRSV' named entity 'vaccines' named entity 'young children' named entity 'rRT-PCR' named entity 'Thermo Fisher Scientific' named entity 'genome sequence data' named entity 'Primer' named entity 'pathogen' named entity 'clinical setting' named entity 'false positive results' named entity 'real-time RT-PCR'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software