About: RNA-Binding Domain in the Nucleocapsid Protein of Gill-Associated Nidovirus of Penaeid Shrimp

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About: RNA-Binding Domain in the Nucleocapsid Protein of Gill-Associated Nidovirus of Penaeid Shrimp Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	RNA-Binding Domain in the Nucleocapsid Protein of Gill-Associated Nidovirus of Penaeid Shrimp
Creator	Cowley, J Michalski, W Soowannayan, C Walker, P
Source	Medline; PMC
abstract	Gill-associated virus (GAV) infects Penaeus monodon shrimp and is the type species okavirus in the Roniviridae, the only invertebrate nidoviruses known currently. Electrophoretic mobility shift assays (EMSAs) using His(6)-tagged full-length and truncated proteins were employed to examine the nucleic acid binding properties of the GAV nucleocapsid (N) protein in vitro. The EMSAs showed full-length N protein to bind to all synthetic single-stranded (ss)RNAs tested independent of their sequence. The ssRNAs included (+) and (−) sense regions of the GAV genome as well as a (+) sense region of the M RNA segment of Mourilyan virus, a crustacean bunya-like virus. GAV N protein also bound to double-stranded (ds)RNAs prepared to GAV ORF1b gene regions and to bacteriophage M13 genomic ssDNA. EMSAs using the five N protein constructs with variable-length N-terminal and/or C-terminal truncations localized the RNA binding domain to a 50 amino acid (aa) N-terminal sequence spanning Met(11) to Arg(60). Similarly to other RNA binding proteins, the first 16 aa portion of this sequence was proline/arginine rich. To examine this domain in more detail, the 18 aa peptide (M(11)PVRRPLPPQPPRNARLI(29)) encompassing this sequence was synthesized and found to bind nucleic acids similarly to the full-length N protein in EMSAs. The data indicate a fundamental role for the GAV N protein proline/arginine-rich domain in nucleating genomic ssRNA to form nucleocapsids. Moreover, as the synthetic peptide formed higher-order complexes in the presence of RNA, the domain might also play some role in protein/protein interactions stabilizing the helical structure of GAV nucleocapsids.
has issue date	2011-08-03(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0022156
bibo:pmid	21857914
has license	cc-by
sha1sum (hex)	eaf46f27d9c28f8b3c818b0d7969d9188a7e9c11
schema:url	https://doi.org/10.1371/journal.pone.0022156
resource representing a document's title	RNA-Binding Domain in the Nucleocapsid Protein of Gill-Associated Nidovirus of Penaeid Shrimp
has PubMed Central identifier	PMC3153931
has PubMed identifier	21857914
schema:publication	PLoS One
resource representing a document's body	covid:eaf46f27d9c28f8b3c818b0d7969d9188a7e9c11#body_text
is schema:about of	named entity 'RNA' named entity 'shrimp' named entity 'nucleic acids' named entity 'virus' named entity 'proline' named entity 'His' named entity 'Penaeid' named entity 'PENAEID SHRIMP' named entity 'DOMAIN' named entity 'BIND' named entity 'MOURILYAN VIRUS' named entity 'SHRIMP' named entity 'OKAVIRUS' named entity 'INFECTS' named entity 'BACTERIOPHAGE M13' named entity 'IN VITRO' named entity 'CURRENTLY' named entity 'THEIR' named entity 'REGIONS' named entity 'INVERTEBRATE' named entity 'PORTION' named entity 'VARIABLE' named entity 'TO BIND TO' named entity 'TAGGED' named entity 'C-TERMINAL' named entity 'BOUND' named entity 'RNA SEGMENT' named entity 'EMPLOYED' named entity 'PROLINE' named entity 'PENAEUS MONODON' named entity 'RICH' named entity 'NUCLEOCAPSID' named entity 'MET' named entity 'GENOMIC' named entity 'ARG' named entity 'RNA BINDING PROTEINS' named entity 'TYPE' named entity 'GENE' named entity 'GENOME' named entity 'NUCLEIC ACID BINDING' named entity 'GILL' named entity 'SINGLE' named entity 'RNA BINDING DOMAIN' named entity 'SENSE' named entity 'PROTEINS' named entity 'LOCALIZED' named entity 'PEPTIDE' named entity 'AMINO ACID' named entity 'N-TERMINAL ' named entity 'PROTEIN ' named entity 'CRUSTACEAN' named entity 'SSDNA' named entity 'SEQUENCE' named entity 'NUCLEOCAPSID PROTEIN' named entity 'BINDING' named entity 'DOMAIN' named entity 'PROTEIN ' named entity 'ASSOCIATED' named entity '282' named entity 'GILL-ASSOCIATED VIRUS' named entity 'SPECIES' named entity 'INDEPENDENT OF' named entity 'VIRUS' named entity 'ELECTROPHORETIC MOBILITY SHIFT ASSAYS' named entity 'TO EXAMINE' named entity 'TESTED' named entity 'NUCLEIC ACIDS' named entity 'RONIVIRIDAE' named entity 'KNOWN' named entity 'PROPERTIES' named entity 'LIKE' named entity 'USING' named entity 'FOUND' named entity 'N PROTEIN' named entity 'PREPARED' named entity 'REGION' named entity 'LENGTH' named entity 'INCLUDED'

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