About: Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum

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About: Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum
Creator	Patel, Robin Cunningham, Scott Mandrekar, Jayawant Rosenblatt, Jon
Source	PMC
abstract	Objective. We compared laboratory developed real-time PCR assays for detection of Mycoplasma hominis and for detection and differentiation of Ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for M. hominis and Ureaplasma culture. Methods. 283 genitourinary specimens received in the clinical bacteriology laboratory for M. hominis and Ureaplasma species culture were evaluated. Nucleic acids were extracted using the Total Nucleic Acid Kit on the MagNA Pure 2.0. 5 μL of the extracts were combined with 15 μL of each of the two master mixes. Assays were performed on the LightCycler 480 II system. Culture was performed using routine methods. Results. M. hominis PCR detected 38/42 M. hominis culture-positive specimens, as well as 2 that were culture negative (sensitivity, 90.5%; specificity, 99.2%). Ureaplasma PCR detected 139/144 Ureaplasma culture-positive specimens, as well as 9 that were culture negative (sensitivity, 96.5%; specificity, 93.6%). Of the specimens that tested positive for Ureaplasma species, U. urealyticum alone was detected in 33, U. parvum alone in 109, and both in 6. Conclusion. The described PCR assays are rapid alternatives to culture for detection of M. hominis and Ureaplasma species, and, unlike culture, the Ureaplasma assay easily distinguishes U. urealyticum from parvum.
has issue date	2013-03-11(xsd:dateTime)
bibo:doi	10.1155/2013/168742
bibo:pmid	26904723
has license	cc-by
sha1sum (hex)	ea97faa57e408d3c7a5b84dbe3da7ae4feb9992e
schema:url	https://doi.org/10.1155/2013/168742
resource representing a document's title	Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum
has PubMed Central identifier	PMC4745450
has PubMed identifier	26904723
schema:publication	Int J Bacteriol
resource representing a document's body	covid:ea97faa57e408d3c7a5b84dbe3da7ae4feb9992e#body_text
is schema:about of	named entity '96.5' named entity 'OF EACH' named entity 'SPECIMENS' named entity 'TOTAL' named entity 'PURE' named entity 'EVALUATED' named entity 'EXTRACTS' named entity 'UREAPLASMA UREALYTICUM' named entity 'LIGHTCYCLER' named entity 'POSITIVE' named entity 'ROUTINE' named entity 'NUCLEIC ACID' named entity 'CULTURE' named entity '283' named entity 'SUBMITTED' named entity 'TESTED' named entity 'COMBINED' named entity 'REAL-TIME PCR ' named entity 'DETECTED' named entity 'DETECTION' named entity 'SPECIES' named entity 'CLINICAL BACTERIOLOGY' named entity 'EASILY' named entity 'LABORATORY' named entity 'NUCLEIC ACIDS' named entity 'DESCRIBED' named entity 'SPECIFICITY' named entity 'DIFFERENTIATION' named entity 'MAGNA' named entity 'COMPARED' named entity 'RAPID' named entity 'PERFORMED' named entity 'MYCOPLASMA HOMINIS' named entity '480' named entity 'MASTER' named entity 'CULTURE NEGATIVE' named entity 'CONCLUSION' named entity 'UREAPLASMA' named entity 'HOMINIS' named entity 'OBJECTIVE' named entity 'PCR' named entity 'KIT' named entity '139' named entity 'METHODS' named entity 'USING' named entity 'EXTRACTED' named entity 'GENITOURINARY' named entity 'RECEIVED' named entity 'RESULTS' named entity 'SYSTEM' named entity 'ASSAY' covid:arg/ea97faa57e408d3c7a5b84dbe3da7ae4feb9992e named entity 'ATCC' named entity 'PCR' named entity 'Escherichia coli' named entity 'Mycoplasma hominis' named entity 'Ureaplasma' named entity 'bacteriology' named entity 'Ureaplasma' named entity 'Ureaplasma' named entity 'Nucleic acids' named entity 'Ureaplasma' named entity 'U. urealyticum' named entity 'M. hominis' named entity 'real-time PCR' named entity 'M. hominis' named entity 'genitourinary' named entity 'Mollicutes' named entity 'Roche Diagnostics' named entity 'cloned' named entity 'PCR' named entity 'PCR' named entity '95% CI' named entity 'U. parvum' named entity 'U. urealyticum' named entity 'Cloning' named entity 'arginine' named entity 'Ureaplasma'

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