About: Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells
Creator	Matthews, David Evans, Vanessa Heesomʈ, Kate Lam, Yun Lamond, Angus
Source	PMC
abstract	Adenoviruses replicate primarily in the host cell nucleus, and it is well established that adenovirus infection affects the structure and function of host cell nucleoli in addition to coding for a number of nucleolar targeted viral proteins. Here we used unbiased proteomics methods, including high throughput mass spectrometry coupled with stable isotope labeling by amino acids in cell culture (SILAC) and traditional two-dimensional gel electrophoresis, to identify quantitative changes in the protein composition of the nucleolus during adenovirus infection. Two-dimensional gel analysis revealed changes in six proteins. By contrast, SILAC-based approaches identified 351 proteins with 24 proteins showing at least a 2-fold change after infection. Of those, four were previously reported to have aberrant localization and/or functional relevance during adenovirus infection. In total, 15 proteins identified as changing in amount by proteomics methods were examined in infected cells using confocal microscopy. Eleven of these proteins showed altered patterns of localization in adenovirus-infected cells. Comparing our data with the effects of actinomycin D on the nucleolar proteome revealed that adenovirus infection apparently specifically targets a relatively small subset of nucleolar antigens at the time point examined.
has issue date	2009-10-07(xsd:dateTime)
bibo:doi	10.1074/mcp.m900338-mcp200
bibo:pmid	19812395
has license	cc-by-nc
sha1sum (hex)	ea6c3d35646adfcd7e1c55d7d1f0998ba1107d89
schema:url	https://doi.org/10.1074/mcp.m900338-mcp200
resource representing a document's title	Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells
has PubMed Central identifier	PMC2808258
has PubMed identifier	19812395
schema:publication	Mol Cell Proteomics
resource representing a document's body	covid:ea6c3d35646adfcd7e1c55d7d1f0998ba1107d89#body_text
is schema:about of	named entity 'REVEALED' named entity 'REPLICATE' named entity 'INCLUDING' named entity 'FUNCTION' named entity 'TO IDENTIFY' named entity 'HIGH THROUGHPUT' named entity 'STRUCTURE' named entity 'TARGETED' named entity 'HOST CELL' named entity 'COUPLED' named entity 'MASS SPECTROMETRY' named entity 'GEL' named entity 'CELL NUCLEOLI' named entity 'proteins' named entity 'host' named entity 'adenovirus infection' named entity 'protein' named entity 'host' named entity 'Analysis' named entity 'HOST CELL NUCLEUS' named entity 'QUANTITATIVE' named entity 'METHODS' named entity 'PROTEOMICS ANALYSIS' named entity 'ADDITION' named entity 'PROTEOMICS' named entity 'ADENOVIRUSES' named entity 'NUCLEOLUS' named entity 'CELLS' named entity 'ADENOVIRUS' named entity 'HERE' named entity 'CHANGES' named entity 'PROTEIN COMPOSITION' named entity 'VIRAL PROTEINS' named entity 'INFECTED' named entity 'ANALYSIS' named entity 'NUCLEOLUS' named entity 'NUMBER OF' named entity 'TRADITIONAL' named entity 'STABLE ISOTOPE LABELING BY AMINO ACIDS IN CELL CULTURE' named entity 'NUCLEOLAR' named entity 'USED' named entity 'CODING' named entity 'TWO-DIMENSIONAL GEL ELECTROPHORESIS' named entity 'ADENOVIRUS INFECTION' named entity 'PROTEINS' named entity 'ESTABLISHED' named entity 'number' named entity 'coding' named entity 'composition' named entity 'isotope' named entity 'nucleolus' named entity 'fetal calf serum' named entity 'protein' named entity 'cell division' named entity 'viral proteins' named entity 'Nucleolus' named entity 'histone' named entity 'DAPI stain' named entity 'nucleoli' named entity 'adenoviral' named entity 'adenovirus' named entity 'Nucleolus' named entity 'adenovirus infection' named entity 'cell nucleus' named entity 'Adenoviruses' named entity 'proteomics' named entity 'Nucleolus' named entity 'Proteomics' named entity 'nucleoli' named entity 'Invitrogen' named entity 'protein' named entity 'PBS' named entity 'Microscopy' named entity 'nucleoli' named entity 'proteomics' named entity 'metabolism' named entity 'infection'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software