About: Comparison of immunofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell culture

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About: Comparison of immunofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell culture Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of immunofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell culture
Creator	Arbour, Nathalie Sizun, Jacques Talbot, Pierre
Source	Elsevier; Medline; PMC
abstract	Human coronaviruses, with two known serogroups named 229E and OC43, cause up to one third of common colds and may be associated with serious diseases such as nosocomial respiratory infections, enterocolitis, pericarditis and neurological disorders. Reliable methods of detection in clinical samples are needed for a better understanding of their role in pathology. As a first step in the design of such diagnostic procedures, the sensitivities and specificities of two viral diagnostic assays were compared in an experimental cell culture model: an indirect immuno-fluorescence assay using monoclonal antibodies and reverse transcriptase-polymerase chain reaction amplification of viral RNA from infected cells. Immunofluorescence detected human coronaviruses in cells infected at a MOI as low as 10(−2) (log TCID(50)/ml=4.25 for HCV-229E and 2.0 for HCV-OC43; log PFU/ml=4.83 for HCV-229E and 1.84 for HCV-OC43) versus 10(−3) (HCV-OC43) or 10(−4) (HCV-229E) for reverse transcriptase-polymerase chain reaction amplification (log TCID(50)/ml=1.75 for HCV-229E and 1.5 for HCV-OC43; log PFU/ml=2.3 for HCV-229E and 1.34 for HCV-OC43). There were no false positive signals with other human respiratory pathogens: influenza virus, respiratory syncytial virus and adenovirus. Moreover, each assay was coronavirus serogroup-specific. These results demonstrate the potential usefulness of immunofluorescence with monoclonal antibodies and reverse transcriptase-polymerase chain reaction RNA amplification for the rapid detection of human coronaviruses in infected cell cultures. Both methods could be applied to clinical specimens for the diagnosis of human infections.
has issue date	1998-12-10(xsd:dateTime)
bibo:doi	10.1016/s0166-0934(98)00013-5
bibo:pmid	9694322
has license	no-cc
sha1sum (hex)	e92b5c036c9ce25108a60ce3ec7464c78b9a0b4a
schema:url	https://doi.org/10.1016/s0166-0934%2898%2900013-5
resource representing a document's title	Comparison of immunofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell culture
has PubMed Central identifier	PMC7119642
has PubMed identifier	9694322
schema:publication	J Virol Methods
resource representing a document's body	covid:e92b5c036c9ce25108a60ce3ec7464c78b9a0b4a#body_text
is schema:about of	named entity 'monoclonal antibodies' named entity 'signals' named entity 'detection' named entity 'experimental' named entity 'Comparison' named entity 'immunofluorescence' named entity 'RAPID' named entity 'RESPIRATORY INFECTIONS' named entity 'RELIABLE' named entity 'HUMAN' named entity 'COMPARED' named entity 'INFLUENZA VIRUS' named entity 'CLINICAL' named entity 'DIAGNOSIS' named entity 'INFECTIONS' named entity 'MAY BE' named entity 'EXPERIMENTAL CELL' named entity 'UP TO' named entity '3D4' named entity 'DETECTION' named entity 'RNA AMPLIFICATION' named entity '1.5' named entity 'NAMED' named entity '229E' named entity 'VIRAL' named entity 'SPECIMENS' named entity '229E' named entity 'FIRST STEP' named entity 'APPLIED' named entity 'KNOWN' named entity 'UNDERSTANDING' named entity 'AMPLIFICATION' named entity 'OC43' named entity 'TCID' named entity 'IMMUNOFLUORESCENCE' named entity 'DETECTION' named entity 'COMPARISON' named entity 'CORONAVIRUSES' named entity 'CELL CULTURE' named entity 'HUMAN' named entity 'RT-PCR' named entity 'SEROGROUP' named entity 'INFECTED CELL' named entity 'USING' named entity 'VIRAL RNA' named entity 'REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION' named entity 'FALSE POSITIVE' named entity 'DIAGNOSTIC' named entity 'USEFULNESS' named entity 'CORONAVIRUSES' named entity 'LOW' named entity 'ASSAY' named entity 'LOG' named entity 'NEUROLOGICAL DISORDERS' named entity 'CORONAVIRUS' named entity 'CELL CULTURES' named entity 'CAUSE' named entity 'INDIRECT' named entity 'POTENTIAL' named entity 'SIGNALS' named entity 'RESPIRATORY PATHOGENS' named entity 'ROLE' named entity 'HCV' named entity 'PFU' named entity 'THESE' named entity 'COMMON COLDS' named entity 'OC43' named entity 'MONOCLONAL ANTIBODIES' named entity 'PATHOLOGY' named entity 'DIAGNOSTIC PROCEDURES' named entity 'CELLS' named entity 'MONOCLONAL ANTIBODIES' named entity 'METHODS' named entity 'SEROGROUPS' named entity 'PERICARDITIS' named entity 'SPECIFIC' named entity 'FLUORESCENCE ASSAY' named entity 'IMMUNOFLUORESCENCE' named entity 'ASSOCIATED WITH'

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