About: Development of a SYBR Green based real-time PCR assay for detection and quantitation of canine parvovirus in faecal samples

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About: Development of a SYBR Green based real-time PCR assay for detection and quantitation of canine parvovirus in faecal samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a SYBR Green based real-time PCR assay for detection and quantitation of canine parvovirus in faecal samples
Creator	Kumar, Manoj Nandi, Sukdeb
Source	Elsevier; Medline; PMC
abstract	The present study describes the development of SYBR Green based real-time polymerase chain reaction (real-time PCR) for detection and quantitation of canine parvovirus type 2 (CPV 2) in faecal samples of dogs. In this assay, the primers were designed and custom-synthesized based on nucleotide sequence of VP2 gene of CPV 2. A standard curve was plotted using 10-fold serial dilution of standard plasmid DNA and Ct value. The standard curve was found to be linear over a 10(−7) dilution. The real-time PCR results were expressed as the number of DNA copies of CPV 2 per mg of faecal samples and showed range of 1.0 × 10(3) to 7.0 × 10(9) copies of viral DNA per mg of stool samples. The analytical sensitivity of the SYBR Green based real-time PCR was shown to be equivalent to 10 copies. Faecal samples (47) from dogs suspected of CPV 2 infection were analyzed by real-time PCR, haemagglutination (HA) assay and by a conventional PCR and 24, 20 and 22 samples were found positive for CPV 2, respectively. Comparison between the results of three different assays revealed that real-time PCR is more sensitive than HA and conventional PCR and allow the detection of low titers of CPV 2 in infected dogs.
has issue date	2010-06-25(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2010.06.007
bibo:pmid	20599563
has license	no-cc
sha1sum (hex)	e929f901fbdd9d3aab8e8c5da76e40985d9cb1d2
schema:url	https://doi.org/10.1016/j.jviromet.2010.06.007
resource representing a document's title	Development of a SYBR Green based real-time PCR assay for detection and quantitation of canine parvovirus in faecal samples
has PubMed Central identifier	PMC7119530
has PubMed identifier	20599563
schema:publication	J Virol Methods
resource representing a document's body	covid:e929f901fbdd9d3aab8e8c5da76e40985d9cb1d2#body_text
is schema:about of	named entity 'copies' named entity 'real-time PCR' named entity 'number' named entity 'assays' named entity 'assay' named entity 'sensitivity' named entity 'real-time PCR' named entity 'positive' named entity 'faecal samples' named entity 'plotted' named entity 'DNA' named entity 'sensitive' named entity 'SYBR Green' named entity 'faecal samples' named entity 'CONVENTIONAL' named entity 'PLASMID DNA' named entity 'COMPARISON' named entity 'QUANTITATION' named entity 'TYPE 2' named entity 'GENE' named entity 'SUSPECTED' named entity 'LOW' named entity 'VIRAL DNA' named entity 'REAL-TIME POLYMERASE CHAIN REACTION' named entity 'CPV' named entity 'RANGE' named entity 'DNA' named entity 'SYBR GREEN' named entity 'TIME' named entity 'DEVELOPMENT' named entity 'SYNTHESIZED' covid:arg/e929f901fbdd9d3aab8e8c5da76e40985d9cb1d2 named entity 'SYBR GREEN' named entity 'CUSTOM' named entity 'POSITIVE FOR' named entity 'EQUIVALENT' named entity 'RESULTS' named entity 'SERIAL' named entity 'REAL-TIME PCR ' named entity 'SAMPLES' named entity 'QUANTITATION' named entity 'BASED' named entity 'DEVELOPMENT' named entity 'PCR ASSAY' named entity 'CANINE PARVOVIRUS' named entity 'FAECAL' named entity 'CURVE' named entity 'LINEAR' named entity 'USING' named entity 'DETECTION' named entity 'INFECTION' named entity 'REVEALED' named entity 'ANALYTICAL' named entity 'STUDY' named entity 'EXPRESSED' named entity 'INFECTED' named entity 'DETECTION' named entity 'PRIMERS' named entity 'SAMPLES' named entity 'PCR' named entity 'CT VALUE' named entity 'REAL-TIME PCR ' named entity 'COPIES' named entity 'BASED' named entity 'GREEN ' named entity 'NUCLEOTIDE SEQUENCE' named entity 'REAL-TIME PCR' named entity 'STOOL' named entity 'CANINE PARVOVIRUS' named entity 'FAECAL' named entity 'FOLD' named entity 'PRESENT' named entity 'HAEMAGGLUTINATION' named entity 'DILUTION' named entity '927' named entity 'ANALYZED' named entity 'ALLOW' named entity 'ASSAY' named entity 'GREEN ' named entity 'NUMBER OF'

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