About: Evaluation of indirect immunofluorescence antibody test and enzyme-linked immunosorbent assay for the diagnosis of infection by Leishmania infantum in clinically normal and sick cats

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About: Evaluation of indirect immunofluorescence antibody test and enzyme-linked immunosorbent assay for the diagnosis of infection by Leishmania infantum in clinically normal and sick cats Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of indirect immunofluorescence antibody test and enzyme-linked immunosorbent assay for the diagnosis of infection by Leishmania infantum in clinically normal and sick cats
Creator	Papadopoulos, Elias Athanasiou, Labrini Rallis, Timoleon Andreadou, Margarita Chatzis, Manolis Ikonomopoulos, John Kasabalis, Dimitrios Koutinas, Alexandros Leontides, Leonidas Mylonakis, Mathios Saridomichelakis, Manolis
Source	Elsevier; Medline; PMC
abstract	Abstract Cats that live in areas where canine and human leishmaniosis due to Leishmania infantum is endemic may become infected and may develop anti-Leishmania antibodies. In this study 50 clinically normal and 50 cats with cutaneous and/or systemic signs that lived in an endemic area and had been previously examined for infection by L. infantum using PCR in four different tissues were serologically tested for the presence of anti-Leishmania IgG (IFAT and ELISA) and IgM (IFAT). The aim was to compare the results of IFAT, ELISA and PCR and to investigate the possible associations between seropositivity to Leishmania spp and signalment, living conditions, season of sampling, health status of the cats, and seropositivity to other infectious agents. Low concentrations of anti-Leishmania IgG were detected by IFAT in 10% of the cats and by ELISA in 1%, whereas anti-Leishmania IgM were detected by IFAT in 1%. There was disagreement between the results of IFAT and ELISA for anti-Leishmania IgG (P = 0.039) and between all serological tests and PCR (P < 0.001). The diagnostic sensitivity of all serological tests, using PCR as the gold standard, was very low, but ELISA and IFAT for anti-Leishmania IgM had 100% specificity. The diagnostic sensitivity of all serological tests could not be improved by changing the cut-off values. Seropositivity for Leishmania spp was not associated with signalment, living conditions, season of sampling and health status of the cats or with seropositivity to feline leukemia virus, feline immunodeficiency virus, feline coronavirus, Toxoplasma gondii and Bartonella henselae. In conclusion, because of their low sensitivity and very high specificity two of the evaluated serological tests (ELISA for anti-Leishmania IgG and IFAT for anti-Leishmania IgM) may be useless as population screening tests but valuable for diagnosing feline infection by L. infantum.
has issue date	2014-12-31(xsd:dateTime)
bibo:doi	10.1016/j.exppara.2014.10.004
bibo:pmid	25307685
has license	els-covid
sha1sum (hex)	e926c24b7cd48965610ff310005f2ef71fb08799
schema:url	https://doi.org/10.1016/j.exppara.2014.10.004
resource representing a document's title	Evaluation of indirect immunofluorescence antibody test and enzyme-linked immunosorbent assay for the diagnosis of infection by Leishmania infantum in clinically normal and sick cats
has PubMed Central identifier	PMC7094338
has PubMed identifier	25307685
schema:publication	Experimental Parasitology
resource representing a document's body	covid:e926c24b7cd48965610ff310005f2ef71fb08799#body_text
is schema:about of	named entity 'infection' named entity 'sensitivity' named entity 'IgM' named entity 'health' named entity 'health' named entity 'PCR' named entity 'serological tests' named entity 'living conditions' named entity 'compare' named entity 'infection' named entity 'LIVING CONDITIONS' named entity 'SEROPOSITIVITY' named entity 'LIVE' named entity 'AREA' named entity 'ANTIBODIES' named entity 'TESTED FOR' named entity 'WHERE' named entity 'LOW' named entity 'ASSOCIATED WITH' named entity 'POSSIBLE' named entity 'CANINE' named entity 'CATS' named entity 'DISAGREEMENT' named entity 'SEROLOGICAL TESTS' named entity 'BARTONELLA HENSELAE' named entity 'CUTANEOUS' named entity 'DIAGNOSING' named entity 'FELINE CORONAVIRUS' named entity 'INDIRECT IMMUNOFLUORESCENCE' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' covid:arg/e926c24b7cd48965610ff310005f2ef71fb08799 named entity 'FELINE' named entity 'NORMAL' named entity 'AIM' named entity 'ANTIBODY TEST' named entity 'EVALUATION' named entity 'NORMAL' named entity 'CATS' named entity 'INFECTION BY LEISHMANIA INFANTUM' named entity 'DIAGNOSIS' named entity 'LEISHMANIA INFANTUM' named entity 'PREVIOUSLY' named entity 'PRESENCE OF' named entity 'IFAT' named entity 'ASSOCIATIONS' named entity 'VALUES' named entity 'DIFFERENT' named entity 'SPP' named entity 'SIGNS' named entity 'SYSTEMIC' named entity 'SCREENING TESTS' named entity 'THE CUT' named entity 'USING' named entity 'SPECIFICITY' named entity 'TISSUES' named entity 'RESULTS' named entity 'INFECTIOUS AGENTS' named entity 'IGM' named entity 'SICK' named entity 'USELESS' named entity 'CONCENTRATIONS' named entity 'EXAMINED FOR' named entity 'POPULATION' named entity 'SAMPLING' named entity 'L. INFANTUM' named entity 'TO INVESTIGATE' named entity '10%' named entity 'GOLD STANDARD' named entity 'INFECTION' named entity 'PCR' named entity 'VERY HIGH'

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