About: Extracellular cysteines define ectopeptidase (APN, CD13) expression and function

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About: Extracellular cysteines define ectopeptidase (APN, CD13) expression and function Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Extracellular cysteines define ectopeptidase (APN, CD13) expression and function
Creator	Ansorge, Siegfried Arndt, Marco Firla, Beate Frank, Karin Lendeckel, Uwe Thiel, Ute Täger, Michael
source	Elsevier; Medline; PMC
abstract	Abstract Alanyl aminopeptidase (APN) is a surface-bound metallopeptidase that processes the N-terminals of biologically active peptides such as enkephalins, angiotensins, neurokinins, and cytokines. It exerts profound activity on vital processes such as immune response, cellular growth, and blood pressure control. Inhibition of either APN gene expression or its enzymatic activity severely affects leukocyte growth and function. We show here that oxidoreductase-mediated modulations of the cell surface thiol status affect the enzymatic activity of APN. Additional evidence for the pivotal role of extracellular cysteines in the APN molecule was obtained when substitution of any of these six cysteines caused complete loss of surface expression and enzymatic activity. In contrast, the transmembrane Cys24 appears to have no similar function. Enzymatically inactive cysteine mutants were retained in the endoplasmic reticulum as shown by high-resolution imaging and Endoglycosidase H digestion. In the absence of any crystal-structure data, the demonstration that individual extracellular cysteines contribute to APN expression and function appears to be of particular importance. The data are the first to show thiol-dependent modulation of the activity of a typical surface-bound peptidase at the cell surface, probably reflecting a general regulating mechanism. This may relate to various disease processes such as inflammation or malignant transformation.
has issue date	2002-04-01(xsd:dateTime)
bibo:doi	10.1016/s0891-5849(01)00827-9
bibo:pmid	11909693
has license	els-covid
sha1sum (hex)	e8fa052071eb975ce74b51e1927e26803deea863
schema:url	https://doi.org/10.1016/s0891-5849%2801%2900827-9
resource representing a document's title	Extracellular cysteines define ectopeptidase (APN, CD13) expression and function
has PubMed Central identifier	PMC7126538
has PubMed identifier	11909693
schema:publication	Free Radical Biology and Medicine
resource representing a document's body	covid:e8fa052071eb975ce74b51e1927e26803deea863#body_text
is schema:about of	named entity 'EXPRESSION' named entity 'mutants' named entity 'metallopeptidase' named entity 'MODULATION' named entity 'ALANYL AMINOPEPTIDASE' named entity 'PEPTIDASE' named entity 'ENZYMATIC ACTIVITY' named entity 'CONTRIBUTE ' named entity 'PROCESSES' named entity 'LEUKOCYTE' named entity 'REGULATING' named entity 'PARTICULAR' named entity 'VITAL' named entity 'ACTIVITY' named entity 'ENDOGLYCOSIDASE' named entity 'BOUND' named entity 'DIGESTION' named entity 'BIOLOGICALLY' named entity 'HAVE' named entity 'CRYSTAL' named entity 'DATA' named entity 'FUNCTION' named entity 'SEVERELY' named entity 'SIMILAR' named entity 'EXERTS' covid:arg/e8fa052071eb975ce74b51e1927e26803deea863 named entity 'CD13' named entity 'MOLECULE' named entity 'THESE' named entity 'GENERAL' named entity 'CONTRAST' named entity 'STRUCTURE DATA' named entity 'EITHER' named entity 'CYSTEINES' named entity 'EXPRESSION' named entity 'CYTOKINES' named entity 'RETAINED' named entity 'ANGIOTENSINS' named entity 'APN' named entity 'AFFECT' named entity 'GENE EXPRESSION' named entity 'MALIGNANT TRANSFORMATION' named entity 'ROLE' named entity 'ITS' named entity 'PROBABLY' named entity 'HERE' named entity 'IMAGING' named entity 'CELLULAR GROWTH' named entity 'EVIDENCE FOR' named entity 'ABSENCE OF' named entity 'PEPTIDES' named entity 'CAUSED' named entity 'OBTAINED' named entity 'INDIVIDUAL' named entity 'DEMONSTRATION' named entity 'MECHANISM' named entity 'INHIBITION' named entity 'TYPICAL' named entity 'INACTIVE' named entity 'HIGH-RESOLUTION' named entity 'PROFOUND' named entity 'GROWTH' named entity 'ADDITIONAL' named entity 'THIOL' named entity 'NEUROKININS' named entity 'STATUS' named entity 'SURFACE' named entity 'BLOOD PRESSURE CONTROL' named entity 'ACTIVE' named entity 'CYSTEINE' named entity 'DEPENDENT' named entity 'DISEASE' named entity 'IS A' named entity 'EXTRACELLULAR' named entity 'CYSTEINES'

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