About: Active Immunoprophylaxis and Vaccine Augmentations Mediated by a Novel Plasmid DNA Formulation

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About: Active Immunoprophylaxis and Vaccine Augmentations Mediated by a Novel Plasmid DNA Formulation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Active Immunoprophylaxis and Vaccine Augmentations Mediated by a Novel Plasmid DNA Formulation
Creator	Muthumani, Kar Humeau, Laurent Schultheis, Katherine Nguyen, Jacklyn Broderick, Kate Schommer, Nina Smith, Trevor Weiner, David Yung, Bryan
Source	PMC
abstract	Plasmid DNA (pDNA) gene delivery is a highly versatile technology that has the potential to address a multitude of unmet medical needs. Advances in pDNA delivery to host tissue with the employment of in vivo electroporation (EP) have led to significantly enhanced gene expression and the recent demonstration of clinical efficacy with the platform. Building upon this platform, this study reports that enzyme-mediated modification of the muscle tissue extracellular matrix structure at the site of pDNA delivery operates in a synergistic manner with EP to enhance both local and systemic gene expression further. Specifically, administration of chondroitinase ABC (Cho ABC) to the site of intramuscular delivery of pDNA led to transient disruption of chondroitin sulfate scaffolding barrier, permitting enhanced gene distribution and expression across the tissue. The employment of Cho ABC in combination with CELLECTRA(®) intramuscular EP resulted in increased gene expression by 5.5-fold in mice and 17.98-fold in rabbits. The study demonstrates how this protocol can be universally applied to an active prophylaxis platform to increase the in vivo production of functional immunoglobulin G, and to DNA vaccine protocols to permit drug dose sparing. The data indicate the Cho ABC formulation to be of significant value upon combination with EP to drive enhanced gene expression levels in pDNA delivery protocols.
has issue date	2019-04-01(xsd:dateTime)
bibo:doi	10.1089/hum.2018.241
bibo:pmid	30860399
has license	cc-by
sha1sum (hex)	e8dcb73fafb728fc6297e33e0374b7ef8016e1ef
schema:url	https://doi.org/10.1089/hum.2018.241
resource representing a document's title	Active Immunoprophylaxis and Vaccine Augmentations Mediated by a Novel Plasmid DNA Formulation
has PubMed Central identifier	PMC6479233
has PubMed identifier	30860399
schema:publication	Hum Gene Ther
resource representing a document's body	covid:e8dcb73fafb728fc6297e33e0374b7ef8016e1ef#body_text
is schema:about of	named entity 'pDNA' named entity 'manner' named entity 'combination' named entity 'DNA vaccine' named entity 'ABC' named entity 'potential' named entity 'site' named entity 'address' named entity 'systemic' named entity 'Augmentations' named entity 'Formulation' covid:arg/e8dcb73fafb728fc6297e33e0374b7ef8016e1ef named entity 'expression' named entity 'ABC' named entity 'DNA' named entity 'gene' named entity 'tissue' named entity 'muscle tissue' named entity 'gene' named entity 'vivo' named entity 'efficacy' named entity 'vivo' named entity 'pDNA' named entity 'intramuscular' named entity 'gene expression' named entity 'mice' named entity 'DNA vaccine' named entity 'PBS' named entity 'ABC' named entity 'serum' named entity '450 nm' named entity 'Balb/c' named entity 'TAE buffer' named entity 'goat' named entity 'Serum levels' named entity 'CD4+ T-cell' named entity 'ABC' named entity 'substrate' named entity 'isoflurane' named entity 'hyaluronidase' named entity 'plasmid' named entity 'New Zealand White' named entity 'muscle' named entity 'serum' named entity '5.0' named entity 'hyaluronidases' named entity 'PBS' named entity 'skeletal muscle' named entity 'antibody' named entity 'Balb/c' named entity 'multiple nuclei' named entity 'long-term' named entity 'transfection' named entity 'humoral' named entity 'xenogeneic' named entity 'ABC' named entity 'electroporation' named entity 'muscle' named entity 'enzyme' named entity 'kappa light chain' named entity 'chondroitin sulfate' named entity 'PBS' named entity 'vaccine' named entity 'P.R. China' named entity 'ABC' named entity 'intramuscular' named entity 'gene expression' named entity 'gene' named entity 'guinea pigs' named entity 'PBS' named entity 'ABC' named entity 'serum levels' named entity 'ECM'

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